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Recombinant Mouse PGK1 Protein

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SDS-Page: Recombinant Mouse PGK1 Protein [NBP2-52075]
SDS-Page: Recombinant Mouse PGK1 Protein [NBP2-52075]

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Func, PAGE, Bioactivity
Concentration
1 mg/ml

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Recombinant Mouse PGK1 Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-4170 of Mouse PGK1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSLSNK LTLDKLDVKG KRVVMRVDFN VPMKNNQITN NQRIKAAVPS IKFCLDNGAK SVVLMSHLGR PDGVPMPDKY SLEPVAAELK SLLGKDVLFL KDCVGPEVEN ACANPAAGTV ILLENLRFHV EEEGKGKDAS GNKVKAEPAK IDAFRASLSK LGDVYVNDAF GTAHRAHSSM VGVNLPQKAG GFLMKKELNY FAKALESPER PFLAILGGAK VADKIQLINN MLDKVNEMII GGGMAFTFLK VLNNMEIGTS LYDEEGAKIV KDLMSKAEKN GVKITLPVDF VTADKFDENA KTGQATVASG IPAGWMGLDC GTESSKKYAE AVGRAKQIVW NGPVGVFEWE AFARGTKSLM DEVVKATSRG CITIIGGGDT ATCCAKWNTE DKVSHVSTGG GASLELLEGK VLPGVDALSN V
Details of Functionality
Specific activity: > 500 units/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37C.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
PGK1
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
47.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% glycerol, 1 mM DTT
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Mouse PGK1 Protein

  • Cell migration-inducing gene 10 protein
  • EC 2.7.2.3
  • MGC142128
  • MGC8947
  • MIG10
  • PGK1
  • PGKA
  • PGKAMGC117307
  • phosphoglycerate kinase 1
  • Primer recognition protein 2
  • PRP 2
  • PRP2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. WB image submitted by a verified customer review.
Knockout Validated: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha was detected in immersion fixed DFO treated Hela cells (left) but was not detected in HIF-1 knockout HeLa cells (right) using Mouse Anti-human HIF-1 alpha monoclonal antibody (Catalog # NB100-105) at 25 ug/mL for 3 hours at room temperature. Cells were stained using a NorthernLights (TM) 557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei.
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NBP1-33527
Western Blot: HPRT Antibody [NBP1-33527] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: HPRT Antibody [NBP1-33527] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - Paraffin-embedded rat colon. HPRT antibody diluted at 1:500.
(2 Publications)
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Hexokinase 1
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NBP2-67503
Western Blot: Hexokinase 1 Antibody (ST47-05) [NBP2-67503] - Analysis of Hexokinase 1 on different lysates using anti-Hexokinase 1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293 Lane 3: MCF-7 Lane 4: HepG2
Immunocytochemistry/Immunofluorescence: Hexokinase 1 Antibody (ST47-05) [NBP2-67503] - Staining Hexokinase 1 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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beta
2-Microglobulin Antib
NB500-317
Western Blot: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Analysis using the FITC conjugate of NB500-317. Detection (non-reducing conditions) of whole cell lysate of various cell lines Lane 1: RAJI human Burkitt lymphoma cell line Lane 2: EL4 mouse lymphoblastic lymphoma cell line Lane 3: U937 human histiocytic lymphoma cell line.
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Cyclophilin A was detected in immersion fixed PANC-1 human pancreatic carcinoma cell line using Goat Anti-Human/Mouse/Rat Cyclophilin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3589) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern­Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Erythropoietin/EPO
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MEP00B
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RPLP0 Antibody
NBP1-82859
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Immunohistochemistry-Paraffin: RPLP0 Antibody [NBP1-82859] - Staining of human breast shows moderate cytoplasmic positivity in glandular cells.
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Species: Hu
Applications: ICC/IF, IHC, IHC-P
TATA binding
protein TBP Antibody ...
NBP2-59208
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Chromatin Immunoprecipitation Sequencing: TATA binding protein TBP Antibody (C.15200002) [NBP2-59208] - IP'd DNA was analyzed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (A). The IP'd DNA was analyzed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.
Chromatin Immunoprecipitation: TATA binding protein TBP Antibody (C.15200002) [NBP2-59208] - ChIP results obtained with the antibody against TBP. ChIP assays were performed using U2OS cells, the antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes, a region 0.5 and 1 kb upstream of the GAPDH promoter, respectively, and for exon 2 of the myoglobin gene as a negative control. Figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.
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HYPB Antibody
(CL9956)
NBP2-88941
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Immunohistochemistry-Paraffin: HYPB Antibody (CL9956) [NBP2-88941] - Staining of human cervix, uterine shows moderate to strong nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: HYPB Antibody (CL9956) [NBP2-88941] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.
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beta-Glucuronidase/GUS ...
AF6144
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Simple Western lane view shows lysates of HT‑29 human colon adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for beta ‑Glucuronidase/GUSB at approximately 92 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human beta ‑Glucuronidase/GUSB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6144) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
(1 Publication)
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Applications: WB, Simple Western
EIF3B Antibody
NBP2-24571
Western Blot: EIF3B Antibody [NBP2-24571] - analysis of eIF3B in A431 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) NIH 3T3 cell lysate using eIF3B antibody at 0.25 ug/ml, 0.25 ug/ml and 0.05 ug/ml respectively.
Immunohistochemistry-Paraffin: EIF3B Antibody [NBP2-24571] - Formalin-fixed, paraffin-embedded human brain tissue stained with eIF3B antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Simple Western: EIF3B Antibody [NBP2-24571] - Simple Western lane view shows a specific band for EIF3B in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Species: Hu, Mu
Applications: WB, Simple Western, IHC, IHC-P
Lactate
Dehydrogenase A/LDHA
NBP1-48336
Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Increased lactate level detected in the hippocampus of streptozotocin-injected mice fed an HFD (HFD + STZ). Cropped images of LDHA and LDHB in the cortex. Image collected and cropped by Cite Ab from the following publication (Hyperpolarized [1-13C]lactate flux increased in the hippocampal region in diabetic mice. Mol Brain (2019) licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Immunocytochemical analysis of Lactate Dehydrogenase A in HeLa cells
Immunohistochemistry: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Staining of postnatal day 8(P8) Plp-EGFP transgenic mice brain section. Image from verified customer review.
(4 Reviews)
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Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
SDHA Antibody
(JM10-83)
NBP2-67130
Western Blot: SDHA Antibody (JM10-83) [NBP2-67130] - Analysis of SDHA on Jurkat cells lysates using anti-SDHA antibody at 1/500 dilution.
Immunocytochemistry/Immunofluorescence: SDHA Antibody (JM10-83) [NBP2-67130] - Staining SDHA in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: SDHA Antibody (JM10-83) [NBP2-67130] - Analysis of paraffin-embedded zebrafish tissue using anti-SDHA antibody. Counter stained with hematoxylin.
(1 Review)
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Applications: WB, Flow, ICC/IF, IHC, IHC-P

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Bioinformatics

Gene Symbol PGK1