Recombinant Mouse PGK1 His Protein

SDS-Page: Recombinant Mouse PGK1 Protein [NBP2-52075]

Product Discontinued

Recombinant Mouse PGK1 His Protein Summary

• Description: A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-4170 of Mouse PGK1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSLSNK LTLDKLDVKG KRVVMRVDFN VPMKNNQITN NQRIKAAVPS IKFCLDNGAK SVVLMSHLGR PDGVPMPDKY SLEPVAAELK SLLGKDVLFL KDCVGPEVEN ACANPAAGTV ILLENLRFHV EEEGKGKDAS GNKVKAEPAK IDAFRASLSK LGDVYVNDAF GTAHRAHSSM VGVNLPQKAG GFLMKKELNY FAKALESPER PFLAILGGAK VADKIQLINN MLDKVNEMII GGGMAFTFLK VLNNMEIGTS LYDEEGAKIV KDLMSKAEKN GVKITLPVDF VTADKFDENA KTGQATVASG IPAGWMGLDC GTESSKKYAE AVGRAKQIVW NGPVGVFEWE AFARGTKSLM DEVVKATSRG CITIIGGGDT ATCCAKWNTE DKVSHVSTGG GASLELLEGK VLPGVDALSN V

• Details of Functionality: Specific activity > 500 units/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37C.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PGK1
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 47.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Mouse PGK1 His Protein

• Cell migration-inducing gene 10 protein
• EC 2.7.2.3
• MGC142128
• MGC8947
• MIG10
• PGK1
• PGKA
• PGKAMGC117307
• phosphoglycerate kinase 1
• Primer recognition protein 2
• PRP 2
• PRP2

Background

PGK1 is encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PGK1