Genetic Strategies: Western Blot: PGAM5 Antibody [NBP1-92257] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: PGAM5 Antibody [NBP1-92257] - Staining of human colon, liver, testis and tonsil using Anti-PGAM5 antibody NBP1-92257 (A) shows similar protein distribution ...read more
Immunocytochemistry/ Immunofluorescence: PGAM5 Antibody [NBP1-92257] - Staining of human cell line MCF7 shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PGAM5 Antibody [NBP1-92257] - Staining of human colon shows moderate granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: PGAM5 Antibody [NBP1-92257] - Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: PGAM5 Antibody [NBP1-92257] - Staining of human testis shows moderate granular cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: PGAM5 Antibody [NBP1-92257] - Staining of human tonsil shows moderate granular cytoplasmic positivity in germinal center cells
Simple Western: PGAM5 Antibody [NBP1-92257] - Simple Western lane view shows a specific band for PGAM5 in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more
Simple Western: PGAM5 Antibody [NBP1-92257] - Electropherogram image(s) of corresponding Simple Western lane view. PGAM5 antibody was used at 1:60 dilution on RT-4 lysate(s).
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
32 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
FAQs for PGAM5 Antibody (NBP1-92257). (Showing 1 - 2 of 2 FAQ).
I am interested in PGAM5 antibody (NBP1-92257) for use in mouse cells. However, as described in the information on the website and in the data sheet, the reactivity is only for human cells. Furthermore, the images of the western-blot show many nonspecific bands. I wonder if you may send me a small sample of this antibody to test the reactivity of mouse cell lines? If possible, I could test reactivity of its antibody and send images and optimized protocol. Can you please let the customer know the cross reactivity % for this?
The cross reactivity with mouse is 94% and rat is 92% however because we have not tested it we can't guarantee it. As per company policy, we do not provide free samples; however, we do have a program that may be of interest to you called Innovators Reward Program.
Could you please tell me the concentration of your anti-PGAM5 antibody NBP1-92257? It was purchased on 5-14-13. Our batch has been aliquoted, so we no longer have the original tube with the concentration and lot#.
Our current lot of this antibody is at 0.3 mg/mL. It looks like you may have received a previous lot and I have emailed the lab for the concentration of that lot. It is most likely very close to the concentration of the current lot though. If you need to proceed with your studies immediately, I would recommend assuming that your lot is close to 0.3 mg/mL. I have confirmed that the concentration of the lot I suspect you received is also 0.3 mg/mL.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our PGAM5 Antibody and receive a gift card or discount.