PGA5 Antibody (974731) [Unconjugated]

Western blot shows lysates of human stomach cancer tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Pepsinogen A5/PGA5 Monoclonal Antibody (Catalog # MAB8457) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Pepsinogen A5/PGA5 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Pepsinogen A5/PGA5 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human Pepsinogen A5/PGA5 Monoclonal Antibody (Catalog # MAB8457) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intestinal glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western lane view shows lysates of human stomach tissue and human stomach cancer tissue, loaded at 0.2 mg/mL. A specific band was detected for Pepsinogen A5/PGA5 at approximately 48 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Pepsinogen A5/PGA5 Monoclonal Antibody (Catalog # MAB8457) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clone: 974731
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

PGA5 Antibody (974731) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell, HEK293-derived human Pepsinogen A5/PGA5 protein
  Ile16-Ala388
  Accession # P0DJD9
• Specificity: Detects human Pepsinogen A5/PGA5 protein in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.5-25 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PGA5 Antibody (974731) [Unconjugated]

• EC 3.4.23
• EC 3.4.23.1
• pepsin A
• pepsinogen 5, group I (pepsinogen A)
• Pepsinogen A5
• PGA3
• PGA4
• PGA5

Background

Pepsins are aspartic proteases that are synthesized in the gastric mucosa and secreted into the stomach. They are released as zymogens called pepsinogens which are then converted to active pepsins by the acidic pH of gastric juices (1-3). PGA3, PGA4, and PGA5 are human Pepsinogen A isozymogens that differ in sequence by 2­4 amino acid (aa) residues (4, 5). This recombinant human Pepsinogen A corresponds to PGA5. Human Pepsinogen A isozymogens share approximately 56% aa sequence identity with mouse and rat Pepsinogen A isozymogens. Pepsins have optimal activity under conditions of acidic pH and are inhibited by pepstatin (6, 7). Pepsin A has broad substrate specificity, but preferentially cleaves peptide bonds involving aromatic and aliphatic amino acids.

1. Athauda, S.B. et al. (1989) J. Biochem. 106:920.
2. Kageyama, T. et al. (1989) J. Biochem. 105:15.
3. Kageyama, T. (2002) Cell. Mol. Life Sci. 59:288.
4. Zwiers, A. et al. (1994) Clin. Nephrol. 41:153.
5. Nakai, H. et al. (1986) Cytogenet. Cell Genet. 43:215.
6. Marciniszyn, J., Jr. et al. (1976) J. Biol. Chem. 251:7088.
7. Kageyama, T. and K. Takahashi (1980) J. Biochem. 88:571.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P0DJD9()