PGA5 Antibody (974731) [Alexa Fluor™ Plus 405]

• Clone: 974731
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 405

PGA5 Antibody (974731) [Alexa Fluor™ Plus 405] Summary

• Specificity: Detects human Pepsinogen A5/PGA5 protein in direct ELISAs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Pepsins are aspartic proteases that are synthesized in the gastric mucosa and secreted into the stomach. They are released as zymogens called pepsinogens which are then converted to active pepsins by the acidic pH of gastric juices (1-3). PGA3, PGA4, and PGA5 are human Pepsinogen A isozymogens that differ in sequence by 2­4 amino acid (aa) residues (4, 5). This recombinant human Pepsinogen A corresponds to PGA5. Human Pepsinogen A isozymogens share approximately 56% aa sequence identity with mouse and rat Pepsinogen A isozymogens. Pepsins have optimal activity under conditions of acidic pH and are inhibited by pepstatin (6, 7). Pepsin A has broad substrate specificity, but preferentially cleaves peptide bonds involving aromatic and aliphatic amino acids.

1. Athauda, S.B. et al. (1989) J. Biochem. 106:920.
2. Kageyama, T. et al. (1989) J. Biochem. 105:15.
3. Kageyama, T. (2002) Cell. Mol. Life Sci. 59:288.
4. Zwiers, A. et al. (1994) Clin. Nephrol. 41:153.
5. Nakai, H. et al. (1986) Cytogenet. Cell Genet. 43:215.
6. Marciniszyn, J., Jr. et al. (1976) J. Biol. Chem. 251:7088.
7. Kageyama, T. and K. Takahashi (1980) J. Biochem. 88:571.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.