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PGA5 Antibodies

We offer PGA5 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Western Blot. Each PGA5 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PGA5 Antibodies can be used in a variety of model species: Human. Use the list below to choose the PGA5 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PGA5 polyclonal antibodies and browse our PGA5 monoclonal antibody catalog.

Alternate Names for PGA5 Antibodies

anti-PGA5 antibody, anti-EC 3.4.23 antibody, anti-EC 3.4.23.1 antibody, anti-pepsin A antibody, anti-pepsinogen 5, group I (pepsinogen A) antibody, anti-pepsinogen A5 antibody, anti-PGA3 antibody, anti-PGA4 antibody

4 Results for "pga5" in Primary Antibodies

MAB8457

PGA5 Antibody (974731) [Unconjugated]

100 μg

MAB8457

Western blot shows lysates of human stomach cancer tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Pepsinogen A5/PGA5 Monoclonal Antibody (Catalog # MAB8457) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Pepsinogen A5/PGA5 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Pepsinogen A5/PGA5 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human Pepsinogen A5/PGA5 Monoclonal Antibody (Catalog # MAB8457) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intestinal glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western lane view shows lysates of human stomach tissue and human stomach cancer tissue, loaded at 0.2 mg/mL. A specific band was detected for Pepsinogen A5/PGA5 at approximately 48 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Pepsinogen A5/PGA5 Monoclonal Antibody (Catalog # MAB8457) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB8457-SP

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AF8457

PGA5 Antibody [Unconjugated]

100 μg

AF8457

Western blot shows lysates of human stomach tissue. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human Pepsinogen A5/PGA5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8457) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pepsinogen A5/PGA5 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of human stomach tissue and human stomach cancer tissue, loaded at 0.2 mg/mL. A specific band was detected for Pepsinogen A5/PGA5 at approximately 48 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human Pepsinogen A5/PGA5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8457) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF8457-SP

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H00005222-M02

PGA5 Antibody (4G9) - Azide and BSA Free

0.1 mg

H00005222-M02

Western Blot: PGA5 Antibody (4G9) [H00005222-M02] - PGA5 monoclonal antibody (M02), clone 4G9 Analysis of PGA5 expression in HepG2.

Immunocytochemistry/Immunofluorescence: PGA5 Antibody (4G9) [H00005222-M02] - Analysis of monoclonal antibody to PGA5 on HeLa cell . Antibody concentration 10 ug/ml.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP2-94203

PGA5 Antibody - Azide and BSA Free

0.1 ml

NBP2-94203

Western Blot: PGA5 Antibody [NBP2-94203] - Analysis of extracts of various cell lines, using PGA5 at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-94203-0.02ml

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