PEX6 Antibody (S233-8)

Western Blot: PEX6 Antibody (S233-8) [NBP2-42199] - analysis of Rat Brain showing detection of 100 kDa PEX6 protein using Mouse Anti-PEX6 Monoclonal Antibody, Clone S233-8 . Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain. Load: 15 ug . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-PEX6 Monoclonal Antibody at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 100 kDa.

Immunocytochemistry/Immunofluorescence: PEX6 Antibody (S233-8) [NBP2-42199] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-PEX6 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Peroxisome, Peroxisome Membrane, Nucleus. Magnification: 60X.

Product Discontinued

PEX6 Antibody (S233-8) Summary

• Immunogen: Fusion protein amino acids 2-294 (N-terminus) of human PEX6. Rat: 82% identity (243/293 amino acids identical). Mouse: 82% identity (244/294 amino acids identical). <50% identity with other PEX proteins.
• Localization: Cytoplasm , Peroxisome , Peroxisome Membrane
• Specificity: Less then 50% identity with other PEX proteins.Detects approx 100kDa.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PEX6
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of PEX6 Antibody was sufficient for detection of PEX6 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Reactivity Notes

Immunogen displays sequence identity for non-tested species: Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for PEX6 Antibody (S233-8)

• PAF2
• PAF-2peroxisome assembly factor 2
• Peroxin-6
• peroxisomal biogenesis factor 6peroxin-6
• Peroxisomal-type ATPase 1
• peroxisome biogenesis factor 6
• PXAAA1peroxisomal AAA-type ATPase 1

Background

PEX6 encodes a member of the AAA (ATPases associated with diverse cellular activities) family of ATPases. This member is a predominantly cytoplasmic protein, which plays a direct role in peroxisomal protein import and is required for PTS1 (peroxisomal targeting signal 1, a C-terminal tripeptide of the sequence ser-lys-leu) receptor activity. Mutations in this gene cause peroxisome biogenesis disorders of complementation group 4 and complementation group 6. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PEX6
• Uniprot:
  • Q13608(Human)