PEX16 Overexpression Lysate

Images

 
Western Blot: PEX16 Overexpression Lysate (Adult Normal) [NBL1-14300] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PEX16.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

PEX16 Overexpression Lysate Summary

Description

PEX16 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC201115

Accession#: NM_004813

Protein Tag: C-MYC/DDK
Gene
PEX16

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
38.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PEX16 Overexpression Lysate

  • peroxin-16
  • peroxisomal biogenesis factor 16peroxin 16
  • peroxisomal membrane protein PEX16
  • peroxisome biogenesis factor 16

Background

The protein encoded by this gene is an integral peroxisomal membrane protein. An inactivating nonsense mutation localized to this gene was observed in a patient with Zellweger syndrome of the complementation group CGD/CG9. Expression of this gene product morphologically and biochemically restores the formation of new peroxisomes, suggesting a role in peroxisome organization and biogenesis. Alternative splicing has been observed for this gene and two variants have been described. [provided by RefSeq]. Transcript Variant: This variant (1) uses an alternate terminal exon. The encoded isoform (1) is shorter than isoform 2 and has a unique C-terminus. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-86210
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P
NBP1-32925
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-87258
Species: Hu, Mu, Po
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-87185
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-80577
Species: Hu
Applications: ICC/IF, WB
NBP2-94643
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP1-80967
Species: Hu
Applications: ICC/IF, IHC, IHC-P
8090-ZN
Species: Hu
Applications: EnzAct
NBP2-49599
Species: Hu
Applications: IHC, IHC-P, WB
NBP1-57578
Species: Hu
Applications: WB
NBP1-80955
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP1-77122
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
AF3398
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
NB100-1799
Species: Hu, Mu, Pm, Rt
Applications: ICC/IF, IP, WB
NBP3-18137
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, WB
NBP2-33455
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-86321
Species: Hu
Applications: IHC, IHC-P, WB
H00008443-B01P
Species: Hu
Applications: ICC/IF, WB
H00008800-Q01
Species: Hu
Applications: ELISA, AP, PA, WB
NBP2-13750
Species: Hu
Applications: IHC, IHC-P, WB

Publications for PEX16 Lysate (NBL1-14300) (0)

There are no publications for PEX16 Lysate (NBL1-14300).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PEX16 Lysate (NBL1-14300) (0)

There are no reviews for PEX16 Lysate (NBL1-14300). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PEX16 Lysate (NBL1-14300) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional PEX16 Products

Bioinformatics Tool for PEX16 Lysate (NBL1-14300)

Discover related pathways, diseases and genes to PEX16 Lysate (NBL1-14300). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PEX16 Lysate (NBL1-14300)

Discover more about diseases related to PEX16 Lysate (NBL1-14300).
 

Pathways for PEX16 Lysate (NBL1-14300)

View related products by pathway.

PTMs for PEX16 Lysate (NBL1-14300)

Learn more about PTMs related to PEX16 Lysate (NBL1-14300).

Blogs on PEX16

There are no specific blogs for PEX16, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PEX16 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol PEX16