Pet1 Antibody (HL2045) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Pet1 Antibody (HL2045) - Azide and BSA Free [NBP3-25597] - Pet1 antibody [HL2045] detects Pet1 protein at nucleus by immunofluorescent analysis. Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Pet1 stained by Pet1 antibody [HL2045] (NBP3-25597) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .

• Reactivity: Hu
• Applications: ICC/IF
• Clone: HL2045
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Pet1 Antibody (HL2045) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Pet1 Antibody (HL2045) - Azide and BSA Free (NBP3-25597) is a recombinant monoclonal antibody validated for use in ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Full length human Pet1 recombinant protein
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FEV
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Pet1 Antibody (HL2045) - Azide and BSA Free

• FEV (ETS oncogene family)
• FEV (fifth Ewing variant)
• Fifth Ewing variant protein
• HSRNAFEV
• PC12 ETS domain-containing transcription factor 1
• PC12 ETS factor 1
• PET1
• Pet-1
• protein FEV

Background

Pet1 belongs to the ETS transcription factor family. ETS family members have a highly conserved 85-amino acid ETS domain that binds purine-rich DNA sequences. The alanine-rich C-terminus of this gene indicates that it may act as a transcription repressor. This gene is exclusively expressed in neurons of the central serotonin (5-HT) system, a system implicated in the pathogeny of such psychiatric diseases as depression, anxiety, and eating disorders. In some types of Ewing tumors, this gene is fused to the Ewing sarcoma (EWS) gene following chromosome translocations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FEV