PERQ2 Antibody

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Immunoprecipitation: PERQ2 Antibody [NBP2-12812] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

PERQ2 Antibody Summary

Immunogen
The immunogen this antibody was made to, maps to a region between residue 375 and 425 of human GRB10 Interacting GYF protein 2 using the numbering given in entry NP_001096616.1 (GeneID 26058).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GIGYF2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 28585547).
Reviewed Applications
Read 3 Reviews rated 3.7
using
NBP2-12812 in the following applications:

Publications
Read Publication using
NBP2-12812 in the following applications:

  • 1 publication
  • IP
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PERQ2 Antibody

  • DKFZp686J17223
  • FLJ23368
  • GRB10 interacting GYF protein 2
  • GRB10-interacting GYF protein 2
  • GYF domain containing 2
  • GYF2
  • KIAA0642DKFZp686I15154
  • PARK11
  • Parkinson disease (autosomal recessive, early onset) 11
  • PERQ amino acid rich, with GYF domain 2
  • PERQ amino acid rich, with GYF domain 3
  • PERQ amino acid-rich with GYF domain-containing protein 2
  • PERQ2
  • PERQ3
  • TNRC15
  • trinucleotide repeat containing 15
  • Trinucleotide repeat-containing gene 15 protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PERQ2 Antibody (NBP2-12812)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IP, WB.


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ICC/IF
(1)
IP
(1)
WB
(1)
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Human
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Reviews for PERQ2 Antibody (NBP2-12812) (3) 3.73

Average Rating: 3.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using NBP2-12812:
Filter by Applications
IP
(1)
ICC
(1)
WB
(1)
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Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
Immunoprecipitation PERQ2 NBP2-12812
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4
reviewed by:
Verified Customer
IP Human 01/02/2019
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Summary

ApplicationImmunoprecipitation
Sample Testedb-cell
SpeciesHuman
LotA1

Comments

Comments5ug antibody used with 500ug whole cell lysate. L1: 40ug input, L2: IgG, L3: IP (10ul of 50ul elution)
Immunocytochemistry PERQ2 NBP2-12812
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3
reviewed by:
Verified Customer
ICC Human 11/18/2017
View

Summary

ApplicationImmunocytochemistry
Sample Tested fibroblasts
SpeciesHuman
LotA1

Comments

CommentsBlocked 1H 25C in 1X PBS/5% normal serum/0.3% Triton™ X-100
Diluted 1:100 in 1X PBS/1% BSA/0.3% Triton™ X-100, 16 hours at 4C incubation.
2 hours (25C) secondary (1.5:1000 dilution)
Western Blot PERQ2 NBP2-12812
Enlarge
4
reviewed by:
Verified Customer
WB Human 04/15/2016
View

Summary

ApplicationWestern Blot
Sample Testedhuman lymphocytes, whole cell lysate
SpeciesHuman
LotA1

Blocking

Blocking Details5% Milk/TBS-T, 1 hour, room temperature

Primary Anitbody

Dilution Ratio1:1000 in 5% Milk, TBS-T, 16H, 4C

Secondary Antibody

Secondary DescriptionAmersham ECL Rabbit IgG, HRP-linked whole Ab (from donkey)
Secondary Manufacturer Cat#NA934-100UL
Secondary Concentration1:5000

Details

Detection NotesECL, touch exposure

Comments

CommentsExpected band ~190kDA, Several non-specific bands at lower molecular weight. Very strong signal, 40ug whole cell lysate loaded.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PERQ2 Antibody (NBP2-12812) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional PERQ2 Products

Array NBP2-12812

Bioinformatics Tool for PERQ2 Antibody (NBP2-12812)

Discover related pathways, diseases and genes to PERQ2 Antibody (NBP2-12812). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for PERQ2 Antibody (NBP2-12812)

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Blogs on PERQ2

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Contact Information

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Calculators

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Recent Reviews

3.7
5
0
4
2
3
1
2
0
1
0

Verified Customer
01/02/2019
Application: IP
Species: Human

Verified Customer
11/18/2017
Application: ICC
Species: Human

Verified Customer
04/15/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol GIGYF2
Uniprot