PERQ2 Antibody - BSA Free

Immunoprecipitation: PERQ2 Antibody [NBP2-12812] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PERQ2 antibody NBP2-12811 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. PERQ2 was also immunoprecipitated by rabbit anti-PERQ2 antibodies NBP2-12812 and NBP2-12813, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

• Reactivity: Hu
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PERQ2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PERQ2 Antibody - BSA Free (NBP2-12812) is a polyclonal antibody validated for use in WB, ICC/IF and IP. Anti-PERQ2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen this antibody was made to, maps to a region between residue 375 and 425 of human GRB10 Interacting GYF protein 2 using the numbering given in entry NP_001096616.1 (GeneID 26058).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GIGYF2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Reported in scientific literature (PMID: 28585547).
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PERQ2 Antibody - BSA Free

• DKFZp686J17223
• FLJ23368
• GRB10 interacting GYF protein 2
• GRB10-interacting GYF protein 2
• GYF domain containing 2
• GYF2
• KIAA0642DKFZp686I15154
• PARK11
• Parkinson disease (autosomal recessive, early onset) 11
• PERQ amino acid rich, with GYF domain 2
• PERQ amino acid rich, with GYF domain 3
• PERQ amino acid-rich with GYF domain-containing protein 2
• PERQ2
• PERQ3
• TNRC15
• trinucleotide repeat containing 15
• Trinucleotide repeat-containing gene 15 protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PERQ2 Antibody (NBP2-12812)(1)

Publication using NBP2-12812

• Schopp I, Amaya Ramirez C, Debeljak J et al. Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally defined protein complexes. Nat Commun 2017-06-06 [PMID: 28585547] (WB, ICC/IF, IP, Human)

Reviews for PERQ2 Antibody (NBP2-12812) (3)

Average Rating: 3.7

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Human.

reviewed by: Verified Customer IP Human 01/02/2019

Summary

• Application: Immunoprecipitation
• Sample Tested: b-cell
• Species: Human
• Lot: A1

Comments

• Comments: 5ug antibody used with 500ug whole cell lysate. L1: 40ug input, L2: IgG, L3: IP (10ul of 50ul elution)

reviewed by: Verified Customer ICC Human 11/18/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: fibroblasts
• Species: Human
• Lot: A1

Comments

• Comments: Blocked 1H 25C in 1X PBS/5% normal serum/0.3% Triton™ X-100
  Diluted 1:100 in 1X PBS/1% BSA/0.3% Triton™ X-100, 16 hours at 4C incubation.
  2 hours (25C) secondary (1.5:1000 dilution)

reviewed by: Verified Customer WB Human 04/15/2016

Summary

• Application: Western Blot
• Sample Tested: human lymphocytes, whole cell lysate
• Species: Human
• Lot: A1

Bioinformatics

• Gene Symbol: GIGYF2
• Uniprot:
  • NP_001096616.1(Human)