Publications for PERQ2 Antibody (NBP2-12812)(1)
We have publications tested in 1 confirmed species: Human.
We have publications tested in 3 applications: ICC/IF, IP, WB.
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PERQ2 Antibody (NBP2-12812) (3)
Average Rating: 3.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.
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Reviews using NBP2-12812:
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Verified Customer IP
Application Immunoprecipitation Sample Tested b-cell Species Human Lot A1 Comments
Comments 5ug antibody used with 500ug whole cell lysate. L1: 40ug input, L2: IgG, L3: IP (10ul of 50ul elution)
Verified Customer ICC
Application Immunocytochemistry Sample Tested fibroblasts Species Human Lot A1 Comments
Comments Blocked 1H 25C in 1X PBS/5% normal serum/0.3% Triton™ X-100 Diluted 1:100 in 1X PBS/1% BSA/0.3% Triton™ X-100, 16 hours at 4C incubation. 2 hours (25C) secondary (1.5:1000 dilution)
Verified Customer WB
Application Western Blot Sample Tested human lymphocytes, whole cell lysate Species Human Lot A1 Blocking
Blocking Details 5% Milk/TBS-T, 1 hour, room temperature Primary Anitbody
Dilution Ratio 1:1000 in 5% Milk, TBS-T, 16H, 4C Secondary Antibody
Secondary Description Amersham ECL Rabbit IgG, HRP-linked whole Ab (from donkey) Secondary Manufacturer Cat# NA934-100UL Secondary Concentration 1:5000 Details
Detection Notes ECL, touch exposure Comments
Comments Expected band ~190kDA, Several non-specific bands at lower molecular weight. Very strong signal, 40ug whole cell lysate loaded.
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