PERP Antibody (JU04-51)

Western Blot: PERP Antibody (JU04-51) [NBP2-75616] - Analysis of PERP on human skin tissue lysate using anti-PERP antibody at 1/1,000 dilution.

Immunohistochemistry-Paraffin: PERP Antibody (JU04-51) [NBP2-75616] - Analysis of paraffin-embedded mouse small intestine tissue using anti-PERP antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PERP Antibody (JU04-51) [NBP2-75616] - Analysis of paraffin-embedded human skin tissue using anti-PERP antibody. Counter stained with hematoxylin.

Product Discontinued

PERP Antibody (JU04-51) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human PERP aa 144-193 / 193. (SwissProt: Q96FX8 Human; SwissProt: Q9JK95 Mouse)
• Localization: Cell junction. Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PERP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PERP Antibody (JU04-51)

• dJ496H19.1
• KCP1KCP-1
• Keratinocyte-associated protein 1
• KRTCAP11110017A08Rik
• p53 apoptosis effector related to PMP22
• p53 apoptosis effector related to PMP-22
• P53-induced protein PIGPC1
• PERP, TP53 apoptosis effector
• PIGPC1RP3-496H19.1
• THWkeratinocytes associated protein 1
• Transmembrane protein THW

Background

The p53 tumor suppressor activates either cell cycle arrest or apoptosis in response to cellular stress. A novel gene, PERP, is expressed in a p53-dependent manner and at high levels in apoptotic cells compared with G(1)-arrested cells. PERP induction is linked to p53-dependent apoptosis and is directly activated by p53. These data suggest that PERP is a novel effector of p53-dependent apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PERP Antibody (NBP2-75616)(1)

Publication using NBP2-75616

• Dasgupta A, Arneson-Wissink PC, Schmitt RE et al. Anti-cachectic regulator analysis reveals novel Perp-dependent anti-tumorigenic properties of 3-methyladenine in pancreatic cancer JCI insight 2021-12-07 [PMID: 34874916] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: PERP