Peroxiredoxin 6 Antibody [Unconjugated]

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, A20 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Peroxiredoxin 6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3490) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Peroxiredoxin 6 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Western blot shows lysates of HAP1 human near-haploid cell line and Peroxiredoxin 6 knockout HAP1 cell line (KO). Nitrocellulose membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3490) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for Peroxiredoxin 6 at approximately 25 kDa (as indicated) in the parental HAP1 cell line, but is not detectable in knockout HAP1 cell line. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

• Reactivity: Hu, Mu, Rt
• Applications: WB, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Peroxiredoxin 6 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Peroxiredoxin 6
  Met1-Pro224
  Accession # P30041
• Specificity: Detects endogenous human, mouse, and rat Peroxiredoxin 6 on in Western blots. In Western blots, this antibody does not cross-react with recombinant human Peroxiredoxin 1, 2, 3, or 5.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PRDX6
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Peroxiredoxin 6 Antibody [Unconjugated]

• 1-Cys
• 24 kDa protein
• Acidic calcium-independent phospholipase A2
• aiPLA2
• aiPLA21-Cys PRX
• Antioxidant protein 2
• AOP2
• AOP2Non-selenium glutathione peroxidase
• EC 1.11.1.15
• EC 1.11.1.7
• EC 3.1.1.-
• KIAA0106Red blood cells page spot 12
• Liver 2D page spot 40
• MGC46173
• NSGPx
• NSGPx1-Cys peroxiredoxin
• p29
• Peroxiredoxin 6
• peroxiredoxin-6
• PRDX6
• PRX

Background

Human peroxiredoxin-6 (Prx-6) is a 26 kDa, cytosolic antioxidant enzyme that belongs to the 1-Cys class of the THP/ahpC family of proteins. The molecule is 224 amino acids (aa) in length and has but one catalytic cysteine at Cys46. Following an attack on peroxide, Cys46 is oxidized to cysteine sulfenic acid and exists in a stable conformation. Reduced, Prx-6 is a homotetramer. When activated, it apparently forms a covalently-associated homodimer. In addition to glutathione peroxidase activity, Prx-6 is also reported to demonstrate phospholipase A₂ activity. Thus, Prx-6 likely plays a role in phospholipid turnover. The enzyme effect is a function of Ser32 in a GWSWG motif. Human Prx-6 is 93%, 92%, 90%, and 94% aa identical to porcine, rat, mouse, and canine Prx-6, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Peroxiredoxin 6 Antibody (AF3490)(1)

Publication using AF3490

• Macco R, Pelizzoni I, Consonni A, Vitali I, Giacalone G, Martinelli Boneschi F, Codazzi F, Grohovaz F, Zacchetti D Astrocytes acquire resistance to iron-dependent oxidative stress upon proinflammatory activation. J Neuroinflammation, 2013-10-28;10(0):130. 2013-10-28 [PMID: 24160637] (Western Blot, Rat)

Bioinformatics

• Gene Symbol: PRDX6
• Uniprot:
  • P30041()