Peroxiredoxin 6 Antibody [Alexa Fluor® 647]

• Reactivity: Hu, Mu, Rt
• Applications: WB, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

Peroxiredoxin 6 Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human Peroxiredoxin 6
  Met1-Pro224
  Accession # P30041
• Specificity: Detects endogenous human, mouse, and rat Peroxiredoxin 6 on in Western blots. In Western blots, this antibody does not cross-react with recombinant human Peroxiredoxin 1, 2, 3, or 5.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Peroxiredoxin 6 Antibody [Alexa Fluor® 647]

• 1-Cys
• 24 kDa protein
• Acidic calcium-independent phospholipase A2
• aiPLA2
• aiPLA21-Cys PRX
• Antioxidant protein 2
• AOP2
• AOP2Non-selenium glutathione peroxidase
• EC 1.11.1.15
• EC 1.11.1.7
• EC 3.1.1.-
• KIAA0106Red blood cells page spot 12
• Liver 2D page spot 40
• MGC46173
• NSGPx
• NSGPx1-Cys peroxiredoxin
• p29
• Peroxiredoxin 6
• peroxiredoxin-6
• PRDX6
• PRX

Background

Human peroxiredoxin-6 (Prx-6) is a 26 kDa, cytosolic antioxidant enzyme that belongs to the 1-Cys class of the THP/ahpC family of proteins. The molecule is 224 amino acids (aa) in length and has but one catalytic cysteine at Cys46. Following an attack on peroxide, Cys46 is oxidized to cysteine sulfenic acid and exists in a stable conformation. Reduced, Prx-6 is a homotetramer. When activated, it apparently forms a covalently-associated homodimer. In addition to glutathione peroxidase activity, Prx-6 is also reported to demonstrate phospholipase A₂ activity. Thus, Prx-6 likely plays a role in phospholipid turnover. The enzyme effect is a function of Ser32 in a GWSWG motif. Human Prx-6 is 93%, 92%, 90%, and 94% aa identical to porcine, rat, mouse, and canine Prx-6, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.