Human Peroxiredoxin 5 ELISA Kit (Colorimetric)

ELISA: Human Peroxiredoxin 5 ELISA Kit (Colorimetric) [NBP2-60586] - Standard curve.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell Culture
• Standard Curve Range: 3.125 - 200 ng/mL (example only; lot dependent)
• Sensitivity: 1.6 ng/mL (example only; lot dependent)

Human Peroxiredoxin 5 ELISA Kit (Colorimetric) Summary

• Description: This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
• Standard Curve Range: 3.125 - 200 ng/mL (example only; lot dependent)
• Sensitivity: 1.6 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 10.2% (example only; lot dependent)
• Intra-Assay: %CV < 5.4% (example only; lot dependent)
• Spike Recovery: 90 - 114% (example only; lot dependent)
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PRDX5

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Peroxiredoxin 5 ELISA Kit (Colorimetric)

• ACR1
• ACR1Peroxisomal antioxidant enzyme
• Alu corepressor 1
• Alu co-repressor 1
• Antioxidant enzyme B166
• AOEB166
• AOEB166Peroxiredoxin V
• AOPP
• B166
• EC 1.11.1.15
• Liver tissue 2D-page spot 71B
• MGC117264
• MGC142283
• MGC142285
• Peroxiredoxin 5
• peroxiredoxin-5, mitochondrial
• PLPThioredoxin peroxidase PMP20
• PMP20
• PRDX5
• PRDX6
• PRXV
• prx-V
• SBBI10
• Thioredoxin reductase
• TPx type VI

Background

Peroxiredoxin 5 encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. This protein interacts with peroxisome receptor 1. The crystal structure of this protein in its reduced form has been resolved to 1.5 angstrom resolution. This gene uses alternate in-frame translation initiation sites to generate mitochondrial or peroxisomal/cytoplasmic forms. Three transcript variants encoding distinct isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Peroxiredoxin 5 ELISA Kit (NBP2-60586). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: PRDX5