| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5724-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38370-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3774-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89862-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38371-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22124-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59388-50ul | |||
![Immunoprecipitation: Peroxiredoxin 5 Antibody (PSH03-90) [NBP3-32740] - Peroxiredoxin 5 was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32740 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32740 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32740 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-32740 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 43 seconds; ECL](http://images.novusbio.com/fullsize/nbp3-32740_rabbit-peroxiredoxin-5-mab-psh03-90-26820241582610.jpg "Immunoprecipitation: Peroxiredoxin 5 Antibody (PSH03-90) [NBP3-32740] - Peroxiredoxin 5 was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32740 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32740 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32740 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-32740 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 43 seconds; ECL")
![Immunohistochemistry: Peroxiredoxin 5 Antibody (PSH03-90) [NBP3-32740] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Peroxiredoxin 5 antibody (NBP3-32740) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32740) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32740_rabbit-peroxiredoxin-5-mab-psh03-90-298202414262615.jpg)
![Immunocytochemistry/ Immunofluorescence: Peroxiredoxin 5 Antibody (PSH03-90) [NBP3-32740] - Immunocytochemistry analysis of HeLa cells labeling Peroxiredoxin 5 with Rabbit anti-Peroxiredoxin 5 antibody (NBP3-32740) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Peroxiredoxin 5 antibody (NBP3-32740) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32740_rabbit-peroxiredoxin-5-mab-psh03-90-292024111484.jpg)
![Flow Cytometry: Peroxiredoxin 5 Antibody (PSH03-90) [NBP3-32740] - Flow cytometric analysis of HeLa cells labeling Peroxiredoxin 5. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32740, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32740_rabbit-peroxiredoxin-5-mab-psh03-90-29202416372132.jpg)
![Western Blot: Peroxiredoxin 5 Antibody (PSH03-90) [NBP3-32740] - Western blot analysis of Peroxiredoxin 5 on different lysates with Rabbit anti-Peroxiredoxin 5 antibody (NBP3-32740) at 1/50,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: A549 cell lysate (10 µg/Lane) Lane 3: MCF7 cell lysate (10 µg/Lane) Lane 4: Mouse liver tissue lysate (15 µg/Lane) Lane 5: Mouse kidney tissue lysate (15 µg/Lane) Lane 6: Rat liver tissue lysate (15 µg/Lane) Lane 7: Rat kidney tissue lysate (15 µg/Lane) Predicted band size: 22 kDa Observed band size: 17 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32740_rabbit-peroxiredoxin-5-mab-psh03-90-3920248453533.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 5 Antibody [NBP2-38370] - Staining in human fallopian tube and liver tissues using anti-PRDX5 antibody. Corresponding PRDX5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-Immunohistochemistry-Paraffin-NBP2-38370-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Peroxiredoxin 5 Antibody [NBP2-38370] - Staining in human fallopian tube and liver tissues using anti-PRDX5 antibody. Corresponding PRDX5 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: Peroxiredoxin 5 Antibody [NBP2-38370] - Analysis using Anti-PRDX5 antibody NBP2-38370 (A) shows similar pattern to independent antibody NBP2-38371 (B).](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-Western-Blot-NBP2-38370-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 5 Antibody [NBP2-38370] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38370-img0005.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 5 Antibody [NBP2-38370] - Staining of human fallopian tube shows high expression.](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-Immunohistochemistry-Paraffin-NBP2-38370-img0006.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 5 Antibody [NBP2-38370] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-Immunohistochemistry-Paraffin-NBP2-38370-img0007.jpg)
![Western Blot: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A: Hela Whole Cell Lysate Lane B: 293T Whole Cell LysateLane C: Jurkat Whole Cell Lysate Lane D: 293 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDaObserved band size:15 kDa](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-002-Western-Blot-NBP2-89862-img0002.jpg "Western Blot: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A: Hela Whole Cell Lysate Lane B: 293T Whole Cell LysateLane C: Jurkat Whole Cell Lysate Lane D: 293 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDaObserved band size:15 kDa")
![Immunoprecipitation: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg Jurkat Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate 2 uL anti-PRDX5 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody:Anti-PRDX5 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 22 kDa Observed band size: 22 kDa](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-002-Immunoprecipitation-NBP2-89862-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A: PRDX5 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:22 kDa Observed band size:15 kDa](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-002-Knockout-Validated-NBP2-89862-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Staining of PRDX5 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-89862 antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-89862_rabbit-monoclonal-peroxiredoxin-5-antibody-002-235202514492332.png)
![Immunohistochemistry-Paraffin: Peroxiredoxin 5 Antibody [NBP2-38371] - Staining in human fallopian tube and liver tissues using anti-PRDX5 antibody. Corresponding PRDX5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-Immunohistochemistry-Paraffin-NBP2-38371-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Peroxiredoxin 5 Antibody [NBP2-38371] - Staining in human fallopian tube and liver tissues using anti-PRDX5 antibody. Corresponding PRDX5 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: Peroxiredoxin 5 Antibody [NBP2-38371] - Analysis using Anti-PRDX5 antibody NBP2-38371 (A) shows similar pattern to independent antibody NBP2-38370 (B).](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-Western-Blot-NBP2-38371-img0007.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 5 Antibody [NBP2-38371] - Staining of human fallopian tube shows high expression.](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-Immunohistochemistry-Paraffin-NBP2-38371-img0004.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 5 Antibody [NBP2-38371] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-Immunohistochemistry-Paraffin-NBP2-38371-img0005.jpg)
![Western Blot: Peroxiredoxin 5 Antibody (SR1264) [NBP3-22124] - Western blot analysis using NBP3-22124 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22124_rabbit-peroxiredoxin-5-mab-sr1264-572023153122.jpg "Western Blot: Peroxiredoxin 5 Antibody (SR1264) [NBP3-22124] - Western blot analysis using NBP3-22124 in HepG2 cell lysate.")
![Western Blot: Peroxiredoxin 5 Antibody (6A10) [NBP2-59388] - Lane 1: Recombinant PRDX5 protein, Lane 2: MCF-7 cell lysates, Lane 3: A549 cell lysates, Lane 4: HeLa cell lysates, Lane 5: 293T cell lysates, Lane 6: Jurkat cell lysates](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-6A10-Western-Blot-NBP2-59388-img0001.jpg "Western Blot: Peroxiredoxin 5 Antibody (6A10) [NBP2-59388] - Lane 1: Recombinant PRDX5 protein, Lane 2: MCF-7 cell lysates, Lane 3: A549 cell lysates, Lane 4: HeLa cell lysates, Lane 5: 293T cell lysates, Lane 6: Jurkat cell lysates")
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 5 Antibody (6A10) [NBP2-59388] - Analysis of PRDX5 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PRDX5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-6A10-Immunocytochemistry-Immunofluorescence-NBP2-59388-img0002.jpg)
![Western Blot: Peroxiredoxin 5 Antibody [NBP1-58348] - Sample Tissue: Human Fetal Lung Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-Western-Blot-NBP1-58348-img0004.jpg "Western Blot: Peroxiredoxin 5 Antibody [NBP1-58348] - Sample Tissue: Human Fetal Lung Antibody Dilution: 1.0 ug/ml")
![Western Blot: Peroxiredoxin 5 Antibody [NBP1-58348] - Titration: 0.2-1 ug/ml, Positive Control: Human Lung.](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-Western-Blot-NBP1-58348-img0003.jpg)
![Immunoprecipitation:[NBP3-29520]-Detection of human PRDX5 by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PRDX5 antibody used for IP at 6 µg per reaction. PRDX5 was also immunoprecipitated by rabbit anti-PRDX5 antibody. For blotting immunoprecipitated PRDX5, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/nbp3-29520_rabbit-peroxiredoxin-5-pab-231202512101683.jpg "Immunoprecipitation:[NBP3-29520]-Detection of human PRDX5 by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PRDX5 antibody used for IP at 6 µg per reaction. PRDX5 was also immunoprecipitated by rabbit anti-PRDX5 antibody. For blotting immunoprecipitated PRDX5, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![Western Blot: Peroxiredoxin 5 Antibody (5E10D3) [NBP3-16300] - Western blot analysis of extracts of various cell lines, using Peroxiredoxin 5 (PRDX5) Rabbit mAb (NBP3-16300) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10148 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Antibody-5E10D3-Western-Blot-NBP3-16300-img0001.jpg "Western Blot: Peroxiredoxin 5 Antibody (5E10D3) [NBP3-16300] - Western blot analysis of extracts of various cell lines, using Peroxiredoxin 5 (PRDX5) Rabbit mAb (NBP3-16300) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10148 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: Peroxiredoxin 5 Overexpression Lysate (Adult Normal) [NBP2-04918] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Peroxiredoxin 5.](http://images.novusbio.com/fullsize/Peroxiredoxin-5-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04918-img0001.jpg "Western Blot: Peroxiredoxin 5 Overexpression Lysate (Adult Normal) [NBP2-04918] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Peroxiredoxin 5.")