Human Peroxiredoxin 4 ELISA Kit (Colorimetric) Summary
| Description |
Quality control test: Standard curve. PRDX4 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human PRDX4. |
| Standard Curve Range |
78.13 to 5000 pg/mL |
| Sensitivity |
<5000 pg/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 5.02% |
| Intra-Assay |
CV% < 4.95% |
| Spike Recovery |
105.71% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
PRDX4 |
Applications/Dilutions
| Dilutions |
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| Application Notes |
Useful for quantification. |
| Publications |
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Packaging, Storage & Formulations
Kit Components
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Components
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- 96 Well Plate (in a aluminium foil bag with desiccant)Human Peroxiredoxin IV microtiter plate: A plate using break-apart strips coated with amouse monoclonal antibody specific to human Peroxiredoxin IV.
- AV-HRP Dilution Buffer
- AV-HRP: Avidin linked Horseradish Peroxidase (HRP, enzyme).
- Incubation Buffer
- Plate sealers: Adhesive sheet.
- Secondary Antibody (lyophilized): Biotinylated anti human Peroxiredoxin IV antibody
- Standard Protein (lyophilized): Recombinant human Peroxiredoxin IV.
- Standard/Sample/Secondary Antibody Dilution Buffer
- Stop Solution: 1 N solution of sulfuric acid (H2SO4).
- Substrate (Stabilized chromogen): Tetramethylbenzidine (TMB) solution.
- Washing Buffer (20x)
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human Peroxiredoxin 4 ELISA Kit (Colorimetric)
Background
Peroxiredoxin-4 is encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Publications for Peroxiredoxin 4 ELISA Kit (KA2121)(6)
Showing Publications 1 -
6 of 6.
| Publications using KA2121 |
Applications |
Species |
| Huang Y, Wang J, Cai J et al. Targeted homing of CCR2-overexpressing mesenchymal stromal cells to ischemic brain enhances post-stroke recovery partially through PRDX4-mediated blood-brain barrier preservation. Theranostics. 2019-04-04 [PMID: 30613272] |
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| Guo X, Noguchi H, Ishii N et al. The Association of Peroxiredoxin 4 with the Initiation and Progression of Hepatocellular Carcinoma. Antioxid Redox Signal 2018-04-24 [PMID: 29687726] |
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| Ibarrola J, Arrieta V, Sadaba R et al. Galectin-3 downregulates antioxidant peroxiredoxin-4 in human cardiac fibroblasts: a new pathway to induce cardiac damage? Clin Sci (Lond) 2018-04-19 [PMID: 29674526] |
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| Nawata A, Noguchi H, Mazaki Y et al. Overexpression of Peroxiredoxin 4 Affects Intestinal Function in a Dietary Mouse Model of Nonalcoholic Fatty Liver Disease. PLoS One 2016-04-01 [PMID: 27035833] |
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| Nabeshima A, Yamada S, Guo X et al. PEROXIREDOXIN 4 PROTECTS AGAINST NONALCOHOLIC STEATOHEPATITIS AND TYPE 2 DIABETES IN A NONGENETIC MOUSE MODEL. Antioxid Redox Signal. 2013-03-12 [PMID: 23477499] |
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| Guo X, Yamada S, Tanimoto A et al. OVEREXPRESSION OF PEROXIREDOXIN 4 ATTENUATES ATHEROSCLEROSIS IN APOLIPOPROTEIN E KNOCKOUT MICE. Antioxid Redox Signal. 2012-05-02 [PMID: 22548251] |
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Product General Protocols
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FAQs for Peroxiredoxin 4 ELISA Kit (KA2121). (Showing 1 - 1 of 1 FAQs).
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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