Peroxiredoxin 4 Antibody (2C12)

Western Blot: Peroxiredoxin 4 Antibody (2C12) [H00010549-M01] - PRDX4 monoclonal antibody (M01), clone 2C12. Analysis of PRDX4 expression in human liver.

Immunohistochemistry-Paraffin: Peroxiredoxin 4 Antibody (2C12) [H00010549-M01] - Analysis of monoclonal antibody to PRDX4 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.

Western Blot: Peroxiredoxin 4 Antibody (2C12) [H00010549-M01] - Analysis of PRDX4 expression in transfected 293T cell line by PRDX4 monoclonal antibody (M01), clone 2C12.Lane 1: PRDX4 transfected lysate(30.5 KDa).Lane 2: Non-transfected lysate.

Product Discontinued

Peroxiredoxin 4 Antibody (2C12) Summary

• Immunogen: PRDX4 (AAH03609, 51 a.a. ~ 150 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CHFFAGGQVYPGEASRVSVADHSLHLSKAKISKPAPYWEGTAVIDGEFKELKLTDYRGKYLVFFFYPLDFTFVCPTEIIAFGDRLEEFRSINTEVVACSV
• Specificity: PRDX4 - peroxiredoxin 4
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRDX4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA and immunohistochemistry (paraffin).

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Peroxiredoxin 4 Antibody (2C12)

• Antioxidant enzyme AOE372
• AOE372
• AOE37-2PRX-4
• EC 1.11.1.15
• Peroxiredoxin 4
• Peroxiredoxin IV
• peroxiredoxin-4
• PRDX4
• Prx4
• prx-4
• prx-IV
• thioredoxin peroxidase (antioxidant enzyme)
• Thioredoxin peroxidase AO372
• Thioredoxin-dependent peroxide reductase A0372

Background

The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRDX4
• Entrez:
  • 10549(Human)
• OMIM:
  • 606506(Human)
• Uniprot:
  • AAH03609(Human)