Peroxiredoxin 1 Antibody (JF0945)

Western Blot: Peroxiredoxin 1 Antibody (JF0945) [NBP2-67042] - Analysis of Peroxiredoxin 1 on different lysates using anti-Peroxiredoxin 1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: A431

Immunocytochemistry/Immunofluorescence: Peroxiredoxin 1 Antibody (JF0945) [NBP2-67042] - Staining Peroxiredoxin 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Peroxiredoxin 1 Antibody (JF0945) [NBP2-67042] - Analysis of paraffin-embedded human liver cancer tissue using anti-Peroxiredoxin 1 antibody. Counter stained with hematoxylin.

Flow Cytometry: Peroxiredoxin 1 Antibody (JF0945) [NBP2-67042] - Analysis of Hela cells with Peroxiredoxin 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Immunocytochemistry/Immunofluorescence: Peroxiredoxin 1 Antibody (JF0945) [NBP2-67042] - Staining Peroxiredoxin 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Peroxiredoxin 1 Antibody (JF0945) [NBP2-67042] - Analysis of paraffin-embedded human kidney tissue using anti-Peroxiredoxin 1 antibody. Counter stained with hematoxylin.

Product Discontinued

Peroxiredoxin 1 Antibody (JF0945) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within N-terminal human Peroxiredoxin 1. (SwissProt: Q06830 Human; SwissProt: P35700 Mouse)
• Localization: Cytoplasm, Melanosome.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRDX1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Peroxiredoxin 1 Antibody (JF0945)

• EC 1.11.1
• EC 1.11.1.15
• Natural killer cell-enhancing factor A
• natural killer-enhancing factor A
• NKEFA
• NKEF-A
• PAG
• PAGAMSP23
• PAGB
• Peroxiredoxin 1
• peroxiredoxin-1
• PRDX1
• proliferation-associated gene A
• Proliferation-associated gene protein
• PRX1
• PRXI
• TDPX2
• Thioredoxin peroxidase 2
• Thioredoxin-dependent peroxide reductase 2

Background

Peroxiredoxin 1 encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Three transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRDX1