Peroxiredoxin 1 Antibody (477609) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: WB, IP, ICC/IF, KO
• Clone: 477609
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

Peroxiredoxin 1 Antibody (477609) [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human Peroxiredoxin 1
  Met1-Lys199
  Accession # Q06830
• Specificity: Detects endogenous human Peroxiredoxin 1 in Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunoprecipitation
  • Knockout Validated
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Peroxiredoxin 1 Antibody (477609) [Alexa Fluor® 594]

• EC 1.11.1
• EC 1.11.1.15
• Natural killer cell-enhancing factor A
• natural killer-enhancing factor A
• NKEFA
• NKEF-A
• PAG
• PAGAMSP23
• PAGB
• Peroxiredoxin 1
• peroxiredoxin-1
• PRDX1
• proliferation-associated gene A
• Proliferation-associated gene protein
• PRX1
• PRXI
• TDPX2
• Thioredoxin peroxidase 2
• Thioredoxin-dependent peroxide reductase 2

Background

Human Peroxiredoxin1 (Prx-1, also known as Thioredoxin peroxidase 2) is a 22 kDa antioxidant enzyme that belongs to the typical 2-Cys class of the THP/ahpC family of proteins. The molecule is 199 amino acids (aa) in length and has two catalytic cysteines, one at Cys52 and a second at Cys173. Prx-1 is an obligate homodimer. In its inactive state, Prx-1 is apparently noncovalently associated. Upon peroxide binding to Cys52 of subunit 1, the Cys173 of subunit 2 interacts with Cys52 of subunit 1 to complete the antioxidation, generating a disulfide bond between Cys52 and Cys173. Subsequent reduction restores the subunits to the basal state. There are apparently two additional isoforms; one shows a premature truncation after aa 171, while the second shows a deletion of aa 21‑121. Human Prx-1 is 96% aa identical to mouse Prx-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.