PERK Antibody - BSA Free

Western Blot: PERK Antibody [NBP1-78017] - PERK in cell lysates. 300ug PERK over-expressing 293T cell lysate (lanes 1 & 2), or 800ug wild type (Lanes 3 & 4), and PERK knock out (lanes 5 & 6) MEF cell lysate were immunoprecipitated with 15ul anti-PERK, followed by western blotIgG with 1:1000 dilution of anti-PERK in 5% milk/TBST buffer. Lane 1, 293T cells over-expressing Myc-PERK wt, Lane 2, 293T cells over-expressing Myc-PERK K618A. Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.

Immunohistochemistry: PERK Antibody [NBP1-78017] - Analysis of mouse mammary gland samples from lactating mice (L10) with anti-PERK. Positive staining signal observed in wild type mouse sample with anti-PERK staining only (middle image), but not in the knock out mouse sample (right image) and pre-immune serum staining (left image) The anti-PERK was diluted 1:1,000 in 5% goat serum in PBS and allowed to incubate for 2h at room temperature in a humidified chamber

Immunohistochemistry staining of mouse mammary gland samples from lactating mice (L10) with Immunochemical's anti-PERK. Positive staining signal observed in wild type mouse sample with anti-PERK staining only (middle image), but not in the knock out mouse sample (right image) and pre-immune serum staining (left image) The anti-PERK was diluted 1:1,000 in 5% goat serum in PBS and allowed to incubate for 2h at room temperature in a humidified chamber. Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.

• Reactivity: Mu
• Applications: WB, ELISA, ICC/IF, IHC, IP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PERK Antibody - BSA Free Summary

• Description: This antiserum is directed against PERK and reacts with the PERK from mouse tissues
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Background: .
• Immunogen: This whole rabbit serum was prepared by repeated immunizations with a recombinant fusion protein from amino acids 601-1115 of mouse deltaN PERK. (Uniprot: Q9Z2B5)
• Localization: Endoplasmic reticulum (ER)
• Specificity: This antiserum is directed against PERK and reacts with the PERK from mouse tissues.
• Isotype: Serum
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF2AK3
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:4000-1:20000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:1000
  • Immunoprecipitation 10-30ul
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Application Notes: This antiserum has been tested for use in western blotting, immunoprecipitation and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect bands approximately 150kDa by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

This antiserum is directed against PERK and reacts with the PERK from mouse tissues. Reactivity to other species is unknown.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Unpurified

Alternate Names for PERK Antibody - BSA Free

• DKFZp781H1925
• EC 2.7.11.1
• EIF2AK3
• eukaryotic translation initiation factor 2 alpha kinase 3
• eukaryotic translation initiation factor 2-alpha kinase 3
• hsPEK
• PEK
• PEKPERKPancreatic eIF2-alpha kinase
• PERK
• PRKR-like endoplasmic reticulum kinase
• WRS

Background

The PKR-like endoplasmic reticulum kinase (PERK, also known as Eukaryotic translation initiation factor 2-alpha kinase 3) is a type I transmembrane protein localized to the endoplasmic reticulum (ER). PERK consists of an N-terminal ER luminal domain, a membrane-spanning region, and a cytosolic C-terminal serine/threonine kinase domain (1). The luminal domain of PERK is bound to the ER chaperone GRP78 in unstressed cells (2). PERK activation occurs upon accumulation of misfolded proteins and the ER lumen, which triggers GRP78 dissociation from PERK thereby allowing PERK dimerization and autophosphorylation (3, 4). PERK phosphorylates two established targets: the eukaryotic translation initiation factor 2 alpha (eIF2', (1)) and the Nrf2 transcription factor (5). Phosphorylation of eIF2' results in attenuation of translation initiation (6). The translational block also contributes to cell cycle arrest due to loss of the G1 regulatory protein, cyclin D1 (7). PERK-dependent phosphorylation of Nrf2 promotes transcription of phase II detoxifying enzymes which is critically important for elimination of intracellular reactive oxygen species (8). Thus, while inhibiting new protein synthesis and thereby decreasing the ER protein load PERK simultaneously induces expression of genes that help restore cellular redox homeostasis and promote survival.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PERK Antibody (NBP1-78017)(2)

Publications using NBP1-78017

• Degan S, May BL, Jin YJ et al. Co-Treatment of Chloroquine and Trametinib Inhibits Melanoma Cell Proliferation and Decreases Immune Cell Infiltration Frontiers in Oncology 2022-06-30 [PMID: 35847840] (Western Blot, Mouse)
• Chakraborty P, Parikh RY, Choi S et al. Carbon monoxide activates PERK-regulated autophagy to induce immunometabolic reprogramming and boost anti-tumor T cell function Cancer research 2022-04-11 [PMID: 35404405] (WB, Mouse)

Bioinformatics

• Gene Symbol: EIF2AK3
• Entrez:
  • 13666(Mouse)
• Uniprot:
  • Q7TQC8(Mouse)
  • Q9Z2B5(Mouse)