PERK Antibody (5G5)

Images

 
Western Blot: PERK Antibody (5G5) [NBP1-51661] - Western blot analysis using EIF2AK3 mAb against HEK293 (1) and EIF2AK3-hIgGFc transfected HEK293 (2) cell lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
5G5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

PERK Antibody (5G5) Summary

Immunogen
Purified recombinant fragment of human EIF2AK3 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EIF2AK3
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
Theoretical MW
125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for PERK Antibody (5G5)

  • DKFZp781H1925
  • EC 2.7.11.1
  • EIF2AK3
  • eukaryotic translation initiation factor 2 alpha kinase 3
  • eukaryotic translation initiation factor 2-alpha kinase 3
  • hsPEK
  • PEK
  • PEKPERKPancreatic eIF2-alpha kinase
  • PERK
  • PRKR-like endoplasmic reticulum kinase
  • WRS

Background

EIF2AK3, a Translational Control type protein kinase, is believed to mediate phosphorylation of eIF2-alpha in response to malfolded proteins in the endoplasmic reticulum. Diabetes and Wolcott-Rallison syndrome (WRS) are linked to the mutations in the EIF2AK3 gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for PERK Antibody (NBP1-51661)

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Blogs on PERK.

eIF2alpha - a regulator of global translation in response to cellular stress
Eukaryotic initiation factor 2 (eIF2) regulates global protein translation by binding to Met-tRNA and the 40S ribosome to form the pre-initiation complex. eIF2 is a heterotrimer consisting of alpha, beta, and gamma subunits. The 36kDA eIF2a subuni...  Read full blog post.

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Bioinformatics

Gene Symbol EIF2AK3
Entrez