Perilipin Antibody (JM10-96)

Western Blot: Perilipin Antibody (JM10-96) [NBP2-67037] - Western blot analysis of Perilipin on human liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: Perilipin Antibody (JM10-96) [NBP2-67037] - Staining Perilipin A in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Western Blot: Perilipin Antibody (JM10-96) [NBP2-67037] - Analysis of Perilipin A on human liver cells lysates using anti-Perilipin A antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: Perilipin Antibody (JM10-96) [NBP2-67037] - Staining Perilipin A in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Perilipin Antibody (JM10-96) [NBP2-67037] - Staining Perilipin A in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

Perilipin Antibody (JM10-96) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Perilipin aa 470-510 / 522. (SwissProt: O60240 Human; SwissProt: Q8CGN5 Mouse)
• Localization: Endoplasmic reticulum, Lipid droplet.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PLIN1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Perilipin Antibody (JM10-96)

• PERI
• perilipin 1
• Perilipin
• perilipin-1
• PLIN
• PLIN1
• PLINLipid droplet-associated protein

Background

The perilipin family includes lipid droplet-associated protein (perilipin) and adipose differentiation-related protein (adipophilin). Perilipin is a modulator of both adipocyte lipid metabolism and adipophilinis involved in the development and maintenance of adipose tissue. Perilipin acts as a protective coating from the body's natural lipases--such as hormone-sensitive lipase--that break triglycerides into glycerol and free fatty acids for use in metabolism, a process called lipolysis. It is also an important regulator of lipid storage and its expression is elevated in obese animals and humans.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLIN1