Perforin Antibody (PRF1/2468) - Azide and BSA Free

Immunohistochemistry-Paraffin: Perforin Antibody (PRF1/2468) - Azide and BSA Free [NBP2-75737] - Formalin-fixed, paraffin-embedded human Spleen stained with Perforin-1 Mouse Monoclonal Antibody (PRF1/2468).

Protein Array: Perforin Antibody (PRF1/2468) - Azide and BSA Free [NBP2-75737] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (mAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.

• Reactivity: Hu
• Applications: IHC, IHC-P, PA
• Clone: PRF1/2468
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Perforin Antibody (PRF1/2468) - Azide and BSA Free Summary

• Immunogen: Recombinant human Perforin-1 protein fragment (around aa 413-552) (exact sequence is proprietary)
• Localization: Cytoplasmic
• Specificity: Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The expression of perforin is reportedly upregulated in activated CD8+ T-cells, natural killer cells and some CD4+ T-cells.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRF1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1 - 2 ug/mL
  • Protein Array
• Application Notes: Positive Control: HDLM-2 Cells. Spleen Immunohistochemistry-Paraffin: 1-2 ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
• Theoretical MW: 75 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Perforin Antibody (PRF1/2468) - Azide and BSA Free

• Cytolysin
• FLH2
• HPLH2
• HPLH2lymphocyte pore forming protein
• Lymphocyte pore-forming protein
• MGC65093
• P1
• P1PFN1
• perforin 1 (pore forming protein)
• Perforin
• perforin-1
• PFP
• PFPcytolysin
• PRF1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRF1
• Uniprot:
  • P14222()