Perforin Antibody (PRF1/2468) - Azide and BSA Free

Images

 
Immunohistochemistry-Paraffin: Perforin Antibody (PRF1/2468) - Azide and BSA Free [NBP2-75737] - Formalin-fixed, paraffin-embedded human Spleen stained with Perforin-1 Mouse Monoclonal Antibody (PRF1/2468).
Protein Array: Perforin Antibody (PRF1/2468) - Azide and BSA Free [NBP2-75737] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (mAb) (in combination with a ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-P, PA
Clone
PRF1/2468
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

Perforin Antibody (PRF1/2468) - Azide and BSA Free Summary

Immunogen
Recombinant human Perforin-1 protein fragment (around aa 413-552) (exact sequence is proprietary)
Localization
Cytoplasmic
Specificity
Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The expression of perforin is reportedly upregulated in activated CD8+ T-cells, natural killer cells and some CD4+ T-cells.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PRF1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1 - 2 ug/mL
  • Protein Array
Application Notes
Positive Control: HDLM-2 Cells. Spleen Immunohistochemistry-Paraffin: 1-2 ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Perforin Antibody (PRF1/2468) - Azide and BSA Free

  • Cytolysin
  • FLH2
  • HPLH2
  • HPLH2lymphocyte pore forming protein
  • Lymphocyte pore-forming protein
  • MGC65093
  • P1
  • P1PFN1
  • perforin 1 (pore forming protein)
  • Perforin
  • perforin-1
  • PFP
  • PFPcytolysin
  • PRF1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-75737AF350
Alexa Fluor 405 NBP2-75737AF405
Alexa Fluor 488 NBP2-75737AF488
Alexa Fluor 532 NBP2-75737AF532
Alexa Fluor 594 NBP2-75737AF594
Alexa Fluor 647 NBP2-75737AF647
Alexa Fluor 700 NBP2-75737AF700
Alexa Fluor 750 NBP2-75737AF750
Biotin NBP2-75737B
DyLight 350 NBP2-75737UV
DyLight 405 NBP2-75737V
DyLight 488 NBP2-75737G
DyLight 550 NBP2-75737R
DyLight 594 NBP2-75737DL594
DyLight 650 NBP2-75737C
DyLight 680 NBP2-75737FR
DyLight 755 NBP2-75737IR
FITC NBP2-75737F
HRP NBP2-75737H
Janelia Fluor 549 NBP2-75737JF549
Janelia Fluor 646 NBP2-75737JF646

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Bioinformatics Tool for Perforin Antibody (NBP2-75737)

Discover related pathways, diseases and genes to Perforin Antibody (NBP2-75737). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Perforin.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

Perforin a Protective Serial Killer
Secretory granule-mediated cell death is one of the the key mechanisms for elimination of virus-infected and transformed target cells by cytotoxic lymphocytes. Formation of the immunological synapse between an effector and a target cell leads to exocy...  Read full blog post.

Perforin Antibodies Reveal Links to Apoptosis and Immune Response
Perforin, also known as the pore-forming protein, pfp, is a 70 kD cytolytic protein expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It is one of the major effector molecules used by CTL and NK cel...  Read full blog post.

Perforin Antibodies for Detecting Immune System Diseases
 Perforin is a calcium-dependent pore forming cytolytic protein. Perforin is partially homologous to the terminal components of the membrane attack complex of complement and produces pores of up to 20nm in diameter on target membranes. Killer lymphocy...  Read full blog post.

Perforin Antibodies Reveal Links to Apoptosis and Immune Response
Perforin, also known as the pore-forming protein, pfp, is a 70 kD cytolytic protein expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It is one of the major effector molecules used by CTL and NK cel...  Read full blog post.

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Bioinformatics

Gene Symbol PRF1
Uniprot