Perforin Antibody (BOR21)

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Western Blot: Perforin Antibody (BOR21) [NBP1-43106] - Immunoblotting of human PBMC (Lane A) or PBMC stimulated with IL-2 for 3 days (Lane B) lysate; probed with 2 ug/mL Anti-Human Perforin Purified and anti-actin and ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
BOR21
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Perforin Antibody (BOR21) Summary

Immunogen
The immunogen for this antibody was Perforin.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PRF1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
The BOR21 antibody has been tested for use in immunoblotting (WB) of human PBMCs at 1-5ug/ml. It is recommended that this antibody be titrated for optimal performance in the assay of interest.

Reactivity Notes

This antibody is reactive to Human.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Perforin Antibody (BOR21)

  • Cytolysin
  • FLH2
  • HPLH2
  • HPLH2lymphocyte pore forming protein
  • Lymphocyte pore-forming protein
  • MGC65093
  • P1PFN1
  • perforin 1 (pore forming protein)
  • perforin-1
  • PFPcytolysin

Background

The BOR21 antibody clone reacts with human perforin (pore-forming protein, pfp). Perforin is one of the cytolytic mediators present in the cytoplasmic granules of cytotoxic T lymphocytes (CTL) and natural killer cells (NK). Perforin is involved in the killing function by CTLs and NKs and has an important role in the immune response against tumors and virus infections.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Perforin Antibody (NBP1-43106) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Perforin Antibody (NBP1-43106)

Discover related pathways, diseases and genes to Perforin Antibody (NBP1-43106). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for Perforin Antibody (NBP1-43106)

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Blogs on Perforin.

Perforin a Protective Serial Killer
Secretory granule-mediated cell death is one of the the key mechanisms for elimination of virus-infected and transformed target cells by cytotoxic lymphocytes. Formation of the immunological synapse between an effector and a target cell leads to exocy...  Read full blog post.

Perforin Antibodies Reveal Links to Apoptosis and Immune Response
Perforin, also known as the pore-forming protein, pfp, is a 70 kD cytolytic protein expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It is one of the major effector molecules used by CTL and NK cel...  Read full blog post.

Perforin Antibodies for Detecting Immune System Diseases
 Perforin is a calcium-dependent pore forming cytolytic protein. Perforin is partially homologous to the terminal components of the membrane attack complex of complement and produces pores of up to 20nm in diameter on target membranes. Killer lymphocy...  Read full blog post.

Perforin Antibodies Reveal Links to Apoptosis and Immune Response
Perforin, also known as the pore-forming protein, pfp, is a 70 kD cytolytic protein expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It is one of the major effector molecules used by CTL and NK cel...  Read full blog post.

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Bioinformatics

Gene Symbol PRF1