![Western Blot: Perforin Antibody (3B4) [H00005551-M04] - Analysis of PRF1 expression in transfected 293T cell line by PRF1 monoclonal antibody (M04), clone 3B4. Lane 1: PRF1 transfected lysatE (61.4 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Perforin-Antibody-3B4-Western-Blot-H00005551-M04-img0003.jpg "Western Blot: Perforin Antibody (3B4) [H00005551-M04] - Analysis of PRF1 expression in transfected 293T cell line by PRF1 monoclonal antibody (M04), clone 3B4. Lane 1: PRF1 transfected lysatE (61.4 KDa). Lane 2: Non-transfected lysate.")
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | PRF1 (NP_005032.2, 461 a.a. ~ 555 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. WSVRLDFGDVLLATGGPLRLQVWDQDSGRDDDLLGTCDQAPKSGSHEVRCNLNHGHLKFRYHARCLPHLGGGTCLDYVPQMLLGEPPGNRSGAVW |
| Specificity | PRF1 - perforin 1 (pore forming protein) (3B4) |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PRF1 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
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Perforin Antibodies Reveal Links to Apoptosis and Immune Response Perforin, also known as the pore-forming protein, pfp, is a 70 kD cytolytic protein expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It is one of the major effector molecules used by CTL and NK cel... Read full blog post. |
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![Immunoprecipitation: Perforin Antibody (3B4) [H00005551-M04] - Analysis of PRF1 transfected lysate using anti-PRF1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PRF1 rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Perforin-Antibody-3B4-Immunoprecipitation-H00005551-M04-img0001.jpg "Immunoprecipitation: Perforin Antibody (3B4) [H00005551-M04] - Analysis of PRF1 transfected lysate using anti-PRF1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PRF1 rabbit polyclonal antibody.")
![ELISA: Perforin Antibody (3B4) [H00005551-M04] - Detection limit for recombinant GST tagged PRF1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Perforin-Antibody-3B4-ELISA-H00005551-M04-img0004.jpg "ELISA: Perforin Antibody (3B4) [H00005551-M04] - Detection limit for recombinant GST tagged PRF1 is 0.1 ng/ml as a capture antibody.")
