Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 350

Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody [Alexa Fluor® 350] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human PAM
  Phe21-Val817
  Accession # P19021
• Specificity: Detects human PAM in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Peptidylglycine alpha-Amidating Monooxygenase/PAM Antibody [Alexa Fluor® 350]

• PAL
• PAM
• pancreatic peptidylglycine alpha-amidating monooxygenase
• peptidyl alpha-amidating enzyme
• peptidyl-alpha-hydroxyglycine alpha-amidating lyase
• peptidylamidoglycolate lyase
• peptidylglycine 2-hydroxylase
• peptidyl-glycine alpha-amidating monooxygenase
• peptidylglycine alpha-amidating monooxygenase
• peptidylglycine alpha-hydroxylating monooxygenase
• PHM

Background

PAM (Peptidyl-Gly alpha -amidating monooxygenase) is a dual-function, 95‑110 kDa protein that belongs to both the lyase and monooxygenase enzyme families. It is expressed in a variety of endocrine and exocrine glands and catalyzes the conversion of C‑terminal Gly into amidated residues. Human ProPAM is a 953 amino acid (aa) type I transmembrane glycoprotein. It contains an 833 aa extracellular domain (ECD) (aa 31‑863) and an 86 aa cytoplasmic tail. The ECD shows one PHM/monooxygenase region (aa 40‑347) and a PAL/lyase domain (aa 495‑817). Both regions are enzymatically active. Alternative splicing may generate a soluble form (aa 31‑828), and remove aa 388‑494 that link the two embedded enzyme segments. Over aa 21‑817, human PAM is 91% aa identical to mouse PAM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.