Peptide YY Antibody (4) - BSA Free

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Peptide YY Antibody (4) - BSA Free Summary

• Immunogen: Synthetic N-terminal fragment of human PYY(3-36) coupled to carrier.
• Epitope: The epitope lies within residues 3-12 of human PYY
• Specificity: Binds human PYY(3-36). Based on inhibition ELISA, shows no cross-reaction with PYY(1-36) or NPY.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PYY
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: ELISA: NBP2-23584 binds free PYY(3-36) in solution. An ELISA dilution guideline of 1/20,000 applies to PYY(3-36) coated directly at 1 ug/mL. NBP2-23584 as a capture antibody forms a sandwich ELISA antibody pair with biotinylated NBP1-05167 as a detection antibody for measuring PYY(3-36). WB: NBP2-23584 can be used in Western blotting.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Peptide YY Antibody (4) - BSA Free

• Peptide tyrosine tyrosine
• Peptide YY
• Peptide-YY
• PYY
• PYY1
• PYY-I

Background

Peptide tyrosine-tyrosine amide (3-36), or PYY(3-36), is a major metabolite of the gut hormone PYY and is produced by the action of dipeptidyl peptidase IV on PYY in the intestinal brush border and in the circulation . PYY(3-36) is a potent inhibitor of food intake in rats and humans, acting selectively on the Y2 receptor to inhibit orexigenic NPY neurons in the arcuate nucleus, thus disinhibiting anorexigenic POMC neurons. The anorexigenic effect of PYY(3-36) is additive to that of the co-secreted gut hormone GLP-1 .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PYY