Pepsinogen C/PGC/Progastricsin Antibody (3M7P3)

Immunohistochemistry: Pepsinogen C/PGC/Progastricsin Antibody (3M7P3) [NBP3-33346] - Immunohistochemistry analysis of paraffin-embedded Human stomach using Pepsinogen C/PGC/Progastricsin(PG II) Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Western Blot: Pepsinogen C/PGC/Progastricsin Antibody (3M7P3) [NBP3-33346] - Western blot analysis of various lysates using Pepsinogen C/PGC/Progastricsin(PG II) Rabbit mAb at1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC
• Clone: 3M7P3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Pepsinogen C/PGC/Progastricsin Antibody (3M7P3) Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-388 of human Pepsinogen C/PGC/Progastricsin (PG II) (NP_002621.1).
  
  Sequence:
  MKWMVVVLVCLQLLEAAVVKVPLKKFKSIRETMKEKGLLGEFLRTHKYDPAWKYRFGDLSVTYEPMAYMDAAYFGEISIGTPPQNFLVLFDTGSSNLWVPSVYCQSQACTSHSRFNPSESSTYSTNGQTFSLQYGSGSLTGFFGYDTLTVQSIQVPNQEFGLSENEPGTNFVYAQFDGIMGLAYPALSVDEATTAMQGMVQEGALTSPVFSVYLSNQQGSSGGAVVFGGVDSSLYTGQIYWAPVTQELYWQIGIEEFLIGGQASGWCSEGCQAIVDTGTSLLTVPQQYMSALLQATGAQEDEYGQFLVNCNSIQNLPSLTFIINGVEFPLPPSSYILSNNGYCTVGVEPTYLSSQNGQPLWILGDVFLRSYYSVYDLGNNRVGFATAA
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PGC
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 ug/mL
  • Immunohistochemistry 1:100 - 1:400
  • Immunohistochemistry-Paraffin 1:100 - 1:400
  • Western Blot 1:1000 - 1:5000
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Pepsinogen C/PGC/Progastricsin Antibody (3M7P3)

• EC 3.4.23
• EC 3.4.23.3
• FLJ99563
• Gastricsin
• PEPC
• pepsin C
• Pepsinogen C
• pepsinogen group II
• PGC
• PGII
• preprogastricsin
• progastricsin (pepsinogen C)

Background

The Progastricsin gene encodes an aspartic proteinase that belongs to the peptidase family A1. The encoded protein is a digestive enzyme that is produced in the stomach and constitutes a major component of the gastric mucosa. This protein is also secreted into the ser

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PGC