Human Pentraxin 2/SAP ELISA Kit (Colorimetric) Summary
| Description |
APCS (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human APCS. |
| Standard Curve Range |
0.078 to 5.0 ng/mL |
| Sensitivity |
40 pg/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 7.1% |
| Intra-Assay |
CV% < 4.9% |
| Spike Recovery |
95% |
| Sample Volume |
50 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
APCS |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Biotinylated Human Serum Amyloid P Antibody (50x): A 50-fold concentratedbiotinylated polyclonal antibody against SAP.
- Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substratetetramethylbenzidine.
- Human Serum Amyloid P Microplate: A 96-well polystyrene microplate (12 strips of 8wells) coated with a polyclonal antibody against human SAP.
- Human Serum Amyloid P Standard: Human SAP in a buffered protein base(lyophilized).
- MIX Diluent Concentrate (10x): A 10-fold concentrated buffered protein base.
- Sealing Tapes: Pressure-sensitive sealing tapes that can be cut to fit the format of theindividual assay.
- Stop Solution: A 0.5 N hydrochloric acid to stop the chromogen substrate reaction.
- Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate.
- Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human Pentraxin 2/SAP ELISA Kit (Colorimetric)
Background
Serum Amyloid P (SAP) is a non-fibrillar plasma glycoprotein that belongs to the pentraxin family. It is universally found in amyloid deposits and this is probably due to its specific calcium-dependent binding to motifs present on all types of amyloid fibrils. SAP is also found to prevent fibrillar breakdown by enzymes and it is believed that it helps maintains stability of the amyloid deposits (1,2). It has been shown that SAP binds monocytes with high avidity, but does not bind to erythrocytes, NK cells, T lymphocytes or B lymphocytes (3). SAP production can be induced by exposure to IL-1, IL-6 and IFN-beta. The SAP-inducing activity was neutralized by antibodies to each of the recombinant cytokines (4).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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