Human Pentraxin 2/SAP ELISA Kit (Colorimetric)

ELISA: Pentraxin 2/SAP ELISA Kit [Biotin] [KA1841] - Standard curve

• Reactivity: Hu
• Applications: ELISA, NULL
• Suitable Sample Type: Cell Culture Sample, CSF, Plasma, Serum, Urine
• Standard Curve Range: 0.078 to 5.0 ng/mL
• Sensitivity: 40 pg/mL

Human Pentraxin 2/SAP ELISA Kit (Colorimetric) Summary

• Description: APCS (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human APCS.
• Standard Curve Range: 0.078 to 5.0 ng/mL
• Sensitivity: 40 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 7.1%
• Intra-Assay: CV% < 4.9%
• Spike Recovery: 95%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: APCS

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human Pentraxin 2/SAP ELISA Kit (Colorimetric)

• 9.5S alpha-1-glycoprotein
• amyloid P component, serum
• APCS
• MGC88159
• Pentraxin 2
• PTX2
• PTX2serum amyloid P-component
• SAP
• SAPpentaxin-related
• Serum Amyloid P component

Background

Serum Amyloid P (SAP) is a non-fibrillar plasma glycoprotein that belongs to the pentraxin family. It is universally found in amyloid deposits and this is probably due to its specific calcium-dependent binding to motifs present on all types of amyloid fibrils. SAP is also found to prevent fibrillar breakdown by enzymes and it is believed that it helps maintains stability of the amyloid deposits (1,2). It has been shown that SAP binds monocytes with high avidity, but does not bind to erythrocytes, NK cells, T lymphocytes or B lymphocytes (3). SAP production can be induced by exposure to IL-1, IL-6 and IFN-beta. The SAP-inducing activity was neutralized by antibodies to each of the recombinant cytokines (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Pentraxin 2/SAP ELISA Kit (KA1841)(1)

Publication using KA1841

• Rodriguez-Rivera C, Perez-Garcia C, Munoz-Rodriguez JR et al. Proteomic Identification of Biomarkers Associated with Eating Control and Bariatric Surgery Outcomes in Patients with Morbid Obesity. World J Surg. 2019-01-01 [PMID: 30426189]

FAQs for Pentraxin 2/SAP ELISA Kit (KA1841). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: APCS