PDK4 Antibody

Product Discontinued

PDK4 Antibody Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PDK4 (AAH40239.1, 1 a.a. - 411 a.a.) full-length recombinant protein with GST tag. MKAARFVLRSAGSLNGAGLVPREVEHFSRYSPSPLSMKQLLDFGSENACERTSFAFLRQELPVRLANILKEIDILPTQLVNTSSVQLVKSWYIQSLMDLVEFHEKSPDDQKALSDFVDTLIKVRNRHHNVVPTMAQGIIEYKDACTVDPVTNQNLQYFLDRFYMNRISTRMLMNQHILIFSDSQTGNPSHIGSIDPNCDVVAVVQDAFECSRMLCDQYYLSSPELKLTQVNGKFPDQPIHIVYVPSHLHHMLFELFKNAMRATVEHQENQPSLTPIEVIVVLGKEDLTIKISDRGGGVPLRIIDRLFSYTYSTAPTPVMDNSRNAPLAGFGYGLPISRLYAKYFQGDLNLYSLSGYGTDAIIYLKALSSESIEKLPVFNKSAFKHYQMSSEADDWCIPSREPKNLAKEVAM
• Specificity: PDK4 - pyruvate dehydrogenase kinase, isoenzyme 4
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: PDK4
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: The quality control of this antibody is limited to WB on the immunizing protein. It has been used for ELISA. Abnova's recommended working dilutions for western analysis are as follows: 1:500 dilution for ascites 1:1000 for purified Ig 1:500

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera with 50% Glycerol
• Preservative: No Preservative
• Purity: Unpurified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PDK4 Antibody

• [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial
• EC 2.7.11
• EC 2.7.11.2
• FLJ40832
• Pyruvate dehydrogenase kinase isoform 4
• pyruvate dehydrogenase kinase, isoenzyme 4
• pyruvate dehydrogenase kinase, isozyme 4
• pyruvate dehydrogenase, lipoamide, kinase isozyme 4, mitochondrial

Background

This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDK4 Antibody (H00005166-A02)(6)

Publications using H00005166-A02

• Laurens C, Badin P, Louche K et al. G0/G1 Switch Gene 2 controls adipose triglyceride lipase activity and lipid metabolism in skeletal muscle. Mol Metab 2016-04-22 [PMID: 27408777]
• Barquissau V, Beuzelin D, Pisani DF et al. White-to-brite conversion in human adipocytes promotes metabolic reprogramming towards fatty acid anabolic and catabolic pathways. Mol Metab 2016-03-18 [PMID: 27110487]
• Kase ET, Nikolic N, Bakke SS et al. Remodeling of oxidative energy metabolism by galactose improves glucose handling and metabolic switching in human skeletal muscle cells. PLoS One. 2013-01-01 [PMID: 23560061] (Human)
• Ribet C, Montastier E, Valle C et al. Peroxisome Proliferator-Activated Receptor-{alpha} Control of Lipid and Glucose Metabolism in Human White Adipocytes. Endocrinology 2009-11-01 [PMID: 19887568]
• Stenson BM, Ryden M, Steffensen KR et al. Activation of liver X receptor (LXR) regulates substrate oxidation in white adipocytes. Endocrinology. 2009-06-25 [PMID: 19556420]
• Laurens C, Badin P, Louche K et al. G0/G1 Switch Gene 2 controls adipose triglyceride lipase activity and lipid metabolism in skeletal muscle. Molecular Metabolism Apr 22 2016

FAQs for PDK4 Antibody (H00005166-A02). (Showing 1 - 1 of 1 FAQs).

1. Which PDK4 antibody do you recommend for immunoblot? I'm working with human cell lines.
   • NBP1-07047 and NBP1-07049 are our best selling PDK4 antibodies that will meet your mentioned criteria i.e. Immunoblot/Western Blot in Human samples. However, all of our products are 100% guaranteed and you may find a complete list of PDK4 antibodies by searching PDK4 on our website and narrowing your results using the filters on the left-hand side of the screen.

Bioinformatics

• Gene Symbol: PDK4
• Entrez:
  • 5166(Human)
• OMIM:
  • 602527(Human)
• Uniprot:
  • AAH40239(Human)