PDIA6 Antibody

Western Blot: PDIA6 Antibody [NBP2-19750] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE diluted at 1:1000

Immunohistochemistry-Paraffin: PDIA6 Antibody [NBP2-19750] - Paraffin-embedded rat liver. PDIA6 antibody [N1N3] diluted at 1:500.

Immunohistochemistry-Paraffin: PDIA6 Antibody [NBP2-19750] - Paraffin-embedded mouse liver. PDIA6 antibody [N1N3] diluted at 1:500.

Immunoprecipitation: PDIA6 Antibody [NBP2-19750] - Immunoprecipitation of PDIA6 protein from 293T whole cell extracts using 5 ug of PDIA6 antibody [N1N3] . Western blot analysis was performed using PDIA6 antibody [N1N3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

• Reactivity: Hu, Mu, Rt, Po, Bv, Pm, RM
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PDIA6 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human PDIA6. The exact sequence is proprietary.
• Localization: Endoplasmic reticulum lumen (By similarity); Melanosome
• Predicted Species: Porcine (94%), Bovine (94%), Rhesus Macaque (98%), Primate (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDIA6
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (99%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for PDIA6 Antibody

• EC 5.3.4.1
• Endoplasmic reticulum protein 5
• ERp5
• ERP5ER protein 5
• P5TXNDC7
• protein disulfide isomerase family A, member 6
• Protein disulfide isomerase P5
• protein disulfide isomerase-associated 6
• protein disulfide isomerase-related protein
• protein disulfide-isomerase A6
• thioredoxin domain containing 7 (protein disulfide isomerase)
• Thioredoxin domain-containing protein 7

Background

Protein disulfide isomerases (EC 5.3.4.1), such as PDIA6, are endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins (Hayano and Kikuchi, 1995 [PubMed 7590364]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PDIA6
• Uniprot:
  • Q15084(Human)