PDIA6 Antibody - BSA Free

Western Blot: PDIA6 Antibody [NBP1-84798] - Analysis using Anti-PDIA6 antibody NBP1-84798 (A) shows similar pattern to independent antibody NBP2-38213 (B).

Staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.

Analysis in human thyroid gland and skeletal muscle tissues using HPA034653 antibody. Corresponding PDIA6 RNA-seq data are presented for the same tissues.

Staining of human cerebral cortex shows moderate granular cytoplasmic positivity in neurons.

Staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes.

Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.

Staining of human cerebral cortex, endometrium, skeletal muscle and thyroid gland using Anti-PDIA6 antibody HPA034653 (A) shows similar protein distribution across tissues to independent antibody HPA034652 (B).

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PDIA6 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: GWLWTEAGAQSELETALGIGGFGYPAMAAINARKMKFALLKGSFSEQGINEFLRELSFGRGSTAPVGGGAFPTIVEREPWDGRDGELPVEDDIDLSDV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDIA6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  PDIA6 Protein (NBP1-84798PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PDIA6 Antibody - BSA Free

• EC 5.3.4.1
• Endoplasmic reticulum protein 5
• ERp5
• ERP5ER protein 5
• P5TXNDC7
• protein disulfide isomerase family A, member 6
• Protein disulfide isomerase P5
• protein disulfide isomerase-associated 6
• protein disulfide isomerase-related protein
• protein disulfide-isomerase A6
• thioredoxin domain containing 7 (protein disulfide isomerase)
• Thioredoxin domain-containing protein 7

Background

Protein disulfide isomerase (PDIA6) ia an enzyme belonging to the protein disulfide isomerase family. Structurally, it contains 2 thioredoxin domains and its function is to catalyze the rearrangement of -S-S- bonds in proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDIA6