PDHX Antibody (RB15204)

Product Discontinued

PDHX Antibody (RB15204) Summary

• Immunogen: This PDX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from human PDX1.
• Localization: Cellular Location Mitochondrion matrix.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDHX
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10 - 1:50
  • Western Blot 1:1000
• Theoretical MW: 54.122 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Adrenal Whole Tissue Lysate (Adult Whole Liver Cirrhosis)

Reactivity Notes

Predicted to react with Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 0.35 mg/ml
• Purity: Protein A purified

Alternate Names for PDHX Antibody (RB15204)

• Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenasecomplex
• DLDBP
• E3-binding protein
• E3BP
• E3BP1
• E3BPpyruvate dehydrogenase complex, E3-binding protein subunit
• OPDX
• PDHX
• PDX1
• PDX1Lipoyl-containing pyruvate dehydrogenase complex component X
• proX
• proXpyruvate dehydrogenase complex, lipoyl-containing component X
• pyruvate dehydrogenase complex, component X
• pyruvate dehydrogenase protein X component, mitochondrial

Background

PDX1, located in the mitochondrial matrix, is required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex. Eukaryotic pyruvate dehydrogenase complexes are organized about a core consisting of the oligomeric dihydrolipoamide acetyl-transferase, around which are arranged multiple copies of pyruvate dehydrogenase, dihydrolipoamide dehydrogenase and protein X bound by noncovalent bonds. Defects in PDHX are a cause of lacticacidemia. PDX1 belongs to the 2-oxoacid dehydrogenase family and contains 1 lipoyl-binding domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDHX
• Uniprot:
  • O00330(Human)