PDE8B Antibody - BSA Free

Immunoprecipitation: PDE8B Antibody [NBP1-71817] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PDE8B antibody used for WB at 1 ug/ml (A and B) and used for IP at 6 ug/mg lysate. PDE8B was also immunoprecipitated by rabbit anti-PDE8B antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PDE8B Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PDE8B Antibody - BSA Free (NBP1-71817) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 835 and 885 of human Phosphodiesterase 8B using the numbering given in entry NP_003710.1 (GeneID 8622).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDE8B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 5 µg/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PDE8B Antibody - BSA Free

• 3'-5' cyclic nucleotide phosphodiesterase 8B
• ADSD
• Cell proliferation-inducing gene 22 protein
• EC 3.1.4.17
• FLJ11212
• high affinity cAMP-specific and IBMX-insensitive 3'-5'-cyclic phosphodiesterase8B
• HsPDE8B
• PDE8B
• phosphodiesterase 8B
• PIG22
• PPNAD3

Background

Cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of the 5-prime,3-prime-cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. Mammalian PDEs have been grouped into several families based on substrate affinities, inhibitor sensitivities, mode of regulation, and amino acid sequence homologies. The PDE8 family contains high-affinity cAMP-specific, IBMX (3-isobutyl-1-methyl-xanthine)-insensitive PDEs. All PDEs share a conserved C-terminal catalytic region and a variable N-terminal domain that presumably accounts for the distinctive regulatory properties unique to the individual families. PDE8B exhibits cAMP PDE activity which was not inhibited by various PDE inhibitors, including IBMX. The cAMP hydrolytic activity was unaffected by cGMP, and no cGMP PDE hydrolysis has been detected. Northern blot analysis indicated that the 4.2-kb PDE8B mRNA was expressed at the highest levels in thyroid, and at lower levels in brain, spinal cord, and placenta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDE8B
• Uniprot:
  • NP_003710.1(Human)