PDE8A Antibody

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Western Blot: PDE8A Antibody [NB300-643] - Western blot using PDE8A (NB300-641) antibody and PC-PDE8A western blot control.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

PDE8A Antibody Summary

Immunogen
Synthetic peptide (Human) (C terminal).
Specificity
PDE8A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDE8A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunoprecipitation 1:10-1:500
Application Notes
IP: Use at a dilution of 1/250. WB: Use at a dilution of 1/500. Detects a band of approximately 98-102 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Positive Control
PDE8A Lysate (NBL1-14232)

Reactivity Notes

Cross-reacts with Human, Mouse and Rat. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris/Glycine buffer pH 7.5-7.9, stabilizing protein, and glycerol
Preservative
0.02% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 50 ul of distilled water to a final concentration is 1.0 mg/ml.

Alternate Names for PDE8A Antibody

  • cAMP-specific cyclic nucleotide phosphodiesterase 8A
  • EC 3.1.4.17
  • FLJ16150
  • high affinity cAMP-specific and IBMX-insensitive 3'-5'-cyclic phosphodiesterase8A
  • high-affinity cAMP-specific and IBMX-insensitive 3'-5'-cyclic phosphodiesterase8A
  • HsT19550
  • phosphodiesterase 8A

Background

Phosphodiesterase type 8 (PDE8) is one of the many phosphodiesterases that compartmentalize and hydrolyze cAMP into AMP. The cAMP-specific PDE8 family is comprised of 2 genes (PDE8A and PDE8B) each with multiple splice variants generated by RNA splicing and use of alternate initiation sites. PDE8 family is a high affinity cAMP-specific, IBMX sensitive PDE. PDE8 has a significant conserved region of about 270 amino acids common to all PDEs at the carboxy terminal apparently serves as the catalytic domain. The amino-terminal region of this protein is divergent and presumably accounts for the distinctive and regulatory properties unique to the individual PDE families. PDE8A protein showed significant homology to other cAMP-dependent PDEs (23%) within the catalytic domain. PDE8A is widely expressed in various tissues in contrast to PDE8B that is exclusively expressed in thyroid gland. The PDE8A transcripts are found in brain, pancreas, placenta, thyroid, spleen, trachea, prostate, and uterus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PDE8A
Entrez