PDE9A Antibody

Images

 
Western Blot: PDE9A Antibody [NBP1-00641] - WB analysis of PDE9A in HeLa whole cell lysate.
Immunocytochemistry/ Immunofluorescence: PDE9A Antibody [NBP1-00641] - PDE9A antibody was tested in NIH/3T3 cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: PDE9A Antibody [NBP1-00641] - PDE9A antibody was tested in mouse prostate using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

PDE9A Antibody Summary

Immunogen
Sequence from the C-terminal region of PDE9A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDE9A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
  • Immunoprecipitation 1:1000
Application Notes
This PDE9A antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation, and Western Blot, where a band can be seen at ~68 kDa. In ICC/IF, cytoplasmic staining was observed in NIH-3T3 cells.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
PDE9A Peptide (NBP1-00641PEP)

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PDE9A Antibody

  • CGMP-specific 3'-5'-cyclic phosphodiesterase type 9
  • CGMP-specific 3'-5'-cyclic phosphodiesterase type 9, EC 3.1.4.1710phosphodiesterase PDE9A21
  • EC 3.1.4.35
  • FLJ90181
  • high affinity cGMP-specific 3'-5'-cyclic phosphodiesterase 9A
  • HSPDE9A2
  • phosphodiesterase 9A

Background

Cyclic nucleotide phosphodiesterases (PDEs) are enzymes which catalyzes the hydrolysis of cyclic adenosine 30,50-monophosphate (cAMP) and/or cyclic guanosine 30,50-monophosphate (cGMP); and PDE9A (high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A) is a PDE member which hydrolyzes the second messenger cGMP, an important regulator of several critical cellular physiological processes. PDE9A activity gets modulated by metal ions and it can bind 2 divalent metal cations per subunit - site 1 binds zinc ions, whereas, site 2 preferentially associates with magnesium and/or manganese ions. PDE9A is expressed in most of the solid tissues and because of its location as well as contribution to the regulation of steady-state cellular concentrations of cyclic nucleotides, PDE9A has been suggested as a potential candidate for diseases such as bipolar affective disorder and its overexpression has been linked to Down syndrome. Moreover, PDE9A deficient mice fed a high-fat diet have been reported to exhibit a reduced weight gain and fat mass compared with wild-type mice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PDE9A Antibody (NBP1-00641) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-00641AF405
Alexa Fluor 488 Labeled NBP1-00641AF488
Alexa Fluor 647 Labeled NBP1-00641AF647
Alexa Fluor 700 Labeled NBP1-00641AF700
Biotin Labeled NBP1-00641B
DyLight 405 Labeled NBP1-00641V
DyLight 488 Labeled NBP1-00641G
DyLight 550 Labeled NBP1-00641R
DyLight 650 Labeled NBP1-00641C
FITC Labeled NBP1-00641F
HRP Labeled NBP1-00641H

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Bioinformatics

Gene Symbol PDE9A
Entrez