PDE9A Antibody - BSA Free

Western Blot: PDE9A Antibody [NBP1-00641] - WB analysis of PDE9A in HeLa whole cell lysate.

Immunocytochemistry/Immunofluorescence: PDE9A Antibody [NBP1-00641] - PDE9A antibody was tested in NIH/3T3 cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue).

Immunohistochemistry: PDE9A Antibody [NBP1-00641] - PDE9A antibody was tested in mouse prostate using DAB with hematoxylin counterstain.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PDE9A Antibody - BSA Free Summary

• Immunogen: Sequence from the C-terminal region of PDE9A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDE9A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
  • Immunoprecipitation 1:1000
  • Western Blot 1:1000
• Application Notes: This PDE9A antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation, and Western Blot, where a band can be seen at ~68 kDa. In ICC/IF, cytoplasmic staining was observed in NIH-3T3 cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  PDE9A Peptide (NBP1-00641PEP)

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PDE9A Antibody - BSA Free

• CGMP-specific 3'-5'-cyclic phosphodiesterase type 9
• CGMP-specific 3'-5'-cyclic phosphodiesterase type 9, EC 3.1.4.1710phosphodiesterase PDE9A21
• EC 3.1.4.35
• FLJ90181
• high affinity cGMP-specific 3'-5'-cyclic phosphodiesterase 9A
• HSPDE9A2
• PDE9A
• phosphodiesterase 9A

Background

Cyclic nucleotide phosphodiesterases (PDEs) are enzymes which catalyzes the hydrolysis of cyclic adenosine 30,50-monophosphate (cAMP) and/or cyclic guanosine 30,50-monophosphate (cGMP); and PDE9A (high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A) is a PDE member which hydrolyzes the second messenger cGMP, an important regulator of several critical cellular physiological processes. PDE9A activity gets modulated by metal ions and it can bind 2 divalent metal cations per subunit - site 1 binds zinc ions, whereas, site 2 preferentially associates with magnesium and/or manganese ions. PDE9A is expressed in most of the solid tissues and because of its location as well as contribution to the regulation of steady-state cellular concentrations of cyclic nucleotides, PDE9A has been suggested as a potential candidate for diseases such as bipolar affective disorder and its overexpression has been linked to Down syndrome. Moreover, PDE9A deficient mice fed a high-fat diet have been reported to exhibit a reduced weight gain and fat mass compared with wild-type mice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDE9A
• Entrez:
  • 5152(Human)
  • 18585(Mouse)
  • 191569(Rat)