Recombinant Human PDE6D His Protein

SDS-Page: Recombinant Human PDE6D Protein [NBP1-44480] - PDE6D, 18.4 kDa (158aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human PDE6D His Protein Summary

• Description: A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-150 of Human PDE6D
  
  

  Source: E.coli

  Amino Acid Sequence: MSAKDERARE ILRGFKLNWM NLRDAETGKI LWQGTEDLSV PGVEHEARVP KKILKCKAVS RELNFSSTEQ MEKFRLEQKV YFKGQCLEEW FFEFGFVIPN STNTWQSLIE AAPESQMMPA SVLTGNVIIE TKFFDDDLLV STSRVRLFYV GSHHHHHH

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PDE6D
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 18.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 1 mM DTT, 100 mM NaCl
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human PDE6D His Protein

• GMP-PDE delta
• PDE6D
• PDED
• phosphodiesterase 6D, cGMP-specific, rod, delta
• Protein p17
• retinal rod rhodopsin-sensitive cGMP 3'-5'-cyclic phosphodiesterase subunitdelta

Background

Human PDE6D was originally identified as a fourth subunit of rod-specific cGMP phosphodiesterase, PDE6. Catalytically active PDE6 is a heterodimer (alphabeta) that is regulated by two inhibitory gamma subunits. As PDE6D does not modify the catalytic properties of PDEalphabeta, its function is still unresolved. Recombinant human PDE6D, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PDE6D