PDE6A Antibody - BSA Free

Immunohistochemistry-Paraffin: PDE6A Antibody [NB110-40461] - Analysis of anti-PDE6A / PDE6 Alpha antibody with human eye, retina at concentration 10 ng/ml.

• Reactivity: Hu, Mu, Rt, Ha, Pm, Pm, Ca
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.23 mg/ml

PDE6A Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1 month.
• Immunogen: Synthetic 18 amino acid peptide from N-terminus of human PDE6A.
• Epitope: N-Terminus
• Localization: Cell membrane; Lipid-anchor; Cytoplasmic side.
• Specificity: Human PDE6A. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Canine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDE6A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10-15 ug/ml
• Application Notes: IHC: NB110-40461 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity..

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (100%), Bovine (89%), Bat (89%), Equine (83%), Opossum (83%).

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 0.23 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PDE6A Antibody - BSA Free

• CGPR-A
• EC 3.1.4
• EC 3.1.4.35
• GMP-PDE alpha
• PDE V-B1
• PDE6A
• PDEA
• PDEAcGMP phosphodiesterase alpha subunit
• phosphodiesterase 6A, cGMP-specific, rod, alpha
• rod cGMP-specific 3'-5'-cyclic phosphodiesterase subunit alpha
• rod photoreceptor cGMP phosphodiesterase alpha subunit
• RP43

Background

This gene encodes the cyclic-GMP (cGMP)-specific phosphodiesterase 6A alpha subunit, expressed in cells of the retinal rod outer segment. The phosphodiesterase 6 holoenzyme is a heterotrimer composed of an alpha, beta, and two gamma subunits. cGMP is an important regulator of rod cell membrane current, and its dynamic concentration is established by phosphodiesterase 6A cGMP hydrolysis and guanylate cyclase cGMP synthesis. The protein is a subunit of a key phototransduction enzyme and participates in processes of transmission and amplification of the visual signal. Mutations in this gene have been identified as one cause of autosomal recessive retinitis pigmentosa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for PDE6A Antibody (NB110-40461). (Showing 1 - 1 of 1 FAQ).

1. Could you by any chance provide more information on the antibody in immunohistochemistry? The picture shown in human is not clear and does not look specific. We are looking to try this out in frozen sections in dog. If you cannot provide this information, would it be possible to obtain a small aliquot for testing before purchasing your antibody? We have tried the PDE6B antibody and it works very cleanly in dog retina, and wish to add to this for a publication, to get PDE6A staining.
   • PDE6A in dogs shares 95% homology so this antibody is predicted to cross-react with canine samples, however, Novus is not able to offer free samples at this time. Since this antibody is predicted to work in canine samples, if you would like to test an antibody in an untested species and share your results with us, I can recommend our Innovator's Reward(TM) program. Our Innovator’s Reward(TM) program was created to allow researchers the opportunity to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply submit an online review detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Reviews may also be submitted by emailing innovators@novusbio.com.

Bioinformatics

• Gene Symbol: PDE6A
• Entrez:
  • 5145(Human)
• Uniprot:
  • P16499(Human)