PDE11A Antibody

Western Blot: PDE11A Antibody [NB100-61669] - Analysis of PDE11A antibody using this antibody at 0.01ug/ml in Human Prostate lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: PDE11A Antibody [NB100-61669] - (1ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PDE11A Antibody Summary

• Description: Novus Biologicals Goat PDE11A Antibody (NB100-61669) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-NSFKESMEKSSYSD corresponding to internal region according to NP_001070665.1, NP_001070826.1, NP_001070664.1.
• Epitope: NSFKESMEKSSYSD
• Specificity: This antibody is expected to recognise all four reported isoforms (NP_058649.3 ; NP_001070665.1; NP_001070826.1; NP_001070664.1).
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PDE11A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 0.5 - 1.5 ug/ml
• Application Notes: WB: Approx. 60-65 kDa band observed in human skeletal muscle and human prostate lysates (calculated MW of 65.7 kDa band according to NP_001070826.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PDE11A Antibody

• cAMP and cGMP cyclic nucleotide phosphodiesterase 11A
• cAMP and cGMP phosphodiesterase 11A
• cyclic nucleotide phosphodiesterase 11A
• dual 3'-5'-cyclic-AMP and -GMP phosphodiesterase 11A
• EC 3.1.4
• EC 3.1.4.17
• EC 3.1.4.35
• EC 3.1.4.53
• FLJ23693
• MGC133355
• MGC133356
• PDE11A
• PDE11A1
• PDE11A2
• PDE11A3
• phosphodiesterase 11A
• PPNAD2

Background

The 3',5'-cyclic nucleotides cAMP and cGMP function as second messengers in a wide variety of signal transduction pathways. 3',5'-cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of cAMP and cGMP to the corresponding 5'-monophosphates and provide a mechanism to downregulate cAMP and cGMP signaling. This gene encodes a member of the PDE protein superfamily. Mutations in this gene are a cause of Cushing disease and adrenocortical hyperplasia. Multiple transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDE11A
• Entrez:
  • 50940(Human)
• Uniprot:
  • NP_058649.3(Human)