PDCD4 [p Ser457] Antibody

Western Blot: PDCD4 [p Ser457] Antibody [NBP3-13143] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PDCD4 (phospho Ser457) antibody (NBP3-13143) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: PDCD4 [p Ser457] Antibody [NBP3-13143] - PDCD4 (phospho Ser457) antibody detects PDCD4 (phospho Ser457) protein at nucleus in rat intestine by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. PDCD4 (phospho Ser457) antibody (NBP3-13143) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: PDCD4 [p Ser457] Antibody [NBP3-13143] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PDCD4 (phospho Ser457) antibody (NBP3-13143) diluted at 1:1000.

Immunohistochemistry-Paraffin: PDCD4 [p Ser457] Antibody [NBP3-13143] - PDCD4 (phospho Ser457) antibody detects PDCD4 (phospho Ser457) protein at nucleus in mouse spleen by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. PDCD4 (phospho Ser457) antibody (NBP3-13143) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Mu, Rt, Hu, Bv, Ch, RM, Xp
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PDCD4 [p Ser457] Antibody Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Carrier-protein conjugated synthetic peptide surrounding phospho Ser457 of human PDCD4. The exact sequence is proprietary.
• Modification: p Ser457
• Predicted Species: Human (100%), Chicken (100%), Xenopus (100%), Bovine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDCD4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for PDCD4 [p Ser457] Antibody

• H731
• H731nuclear antigen H731
• MGC33046
• MGC33047
• Neoplastic transformation inhibitor protein
• Nuclear antigen H731-like
• PDCD4
• programmed cell death 4 (neoplastic transformation inhibitor)
• programmed cell death protein 4
• Protein 197/15a

Background

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1 responsive promoters by c-Jun.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDCD4