PDCD4 Antibody - BSA Free

Western Blot: PDCD4 Antibody [NBP1-76738] - A-20 cell lysate with PDCD4 antibody at (A) 0.5 and (B) 1ug/ml.

Immunohistochemistry: PDCD4 Antibody - BSA Free [NBP1-76738] - Immunohistochemistry of PDCD4 in mouse brain tissue with PDCD antibody at 2.5 ug/mL.

• Reactivity: Hu, Mu, Rt, Ch
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PDCD4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PDCD4 Antibody - BSA Free (NBP1-76738) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-PDCD4 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human PDCD4. The immunogen is located within the last 50 amino acids of PDCD4. Amino Acid Squence: GRKRFVSEGDGGRLKPESY
• Predicted Species: Chicken (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDCD4
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 0.5-1 ug/ml
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  PDCD4 Peptide (NBP1-76738PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for PDCD4 Antibody - BSA Free

• H731
• H731nuclear antigen H731
• MGC33046
• MGC33047
• Neoplastic transformation inhibitor protein
• Nuclear antigen H731-like
• PDCD4
• programmed cell death 4 (neoplastic transformation inhibitor)
• programmed cell death protein 4
• Protein 197/15a

Background

Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. During this process, the expression patterns of numerous genes are radically altered. One such gene is the programmed cell death protein 4 (PDCD4), whose expression was found to be upregulated in all cell lines following the onset of apoptosis. PDCD4 encodes a tumor suppressor protein whose expression is lost in carcinomas of breast, colon, lung and prostate. It can bind to and inhibit the helicase activity of the eukaryotic translation initiation factor 4A and inhibit the transactivation and transformation mediated by the transcription factor AP-1. The kinase Akt regulates PDCD4 by phosphorylation, decreasing the ability of PDCD4 to interfere with the transactivation of AP-1-responsive promoter by c-Jun. There are two known isoforms of PDCD4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDCD4 Antibody (NBP1-76738)(3)

Publications using NBP1-76738

• Pouliquen DL, Boissard A, Henry C Et al. Lymphoid Organ Proteomes Identify Therapeutic Efficacy Biomarkers Following the Intracavitary Administration of Curcumin in a Highly Invasive Rat Model of Peritoneal Mesothelioma International journal of molecular sciences 2021-08-09 [PMID: 34445271] (IHC-P, Rat)
• Xu X, Jiao X, Song N et al. Role of miR-21 on vascular endothelial cells in the protective effect of renal delayed ischemic preconditioning Mol Med Rep 2017-09-01 [PMID: 28677811] (WB)
• Jia P, Teng J, Zou J et al. Xenon Protects Against Septic Acute Kidney Injury via miR-21 Target Signaling Pathway Crit. Care Med. 2015-04-03 [PMID: 25844699] (WB, Mouse)

Bioinformatics

• Gene Symbol: PDCD4
• Entrez:
  • 27250(Human)
  • 18569(Mouse)
  • 64031(Rat)
• Uniprot:
  • B5ME91(Human)