PCPE-1/PCOLCE Antibody [Unconjugated]

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

PCPE-1/PCOLCE Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human PCPE-1
  Met1-Asp449
  Accession # Q15113
• Specificity: Detects human PCPE-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse PCPE-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PCOLCE
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PCPE-1/PCOLCE Antibody [Unconjugated]

• PCOLCE
• PCPE
• PCPE1
• PCPE-1
• PCPE1Type I procollagen COOH-terminal proteinase enhancer
• procollagen C-endopeptidase enhancer 1
• procollagen C-endopeptidase enhancer
• Procollagen COOH-terminal proteinase enhancer 1
• Procollagen C-proteinase enhancer 1
• procollagen, type 1, COOH-terminal proteinase enhancer
• Type 1 procollagen C-proteinase enhancer protein

Background

PCPE-1 is a 55 kDa extracellular protein that lacks intrinsic enzymatic activity. It functions as a substrate-specific adaptor protein that stimulates C-terminal processing of the fibrillar procollagens I, II and III by PCP (also known as BMP-1/tolloid metalloproteinases). PCPE is naturally processed into N-terminal fragments of 34 and 36 kDas, both of which also enhance PCP. A 16.5 kDa protein corresponding to a C-terminal fragment of PCPE has been described and shown to have metalloproteinase inhibitor activity. Human PCPE-1 shares approximately 81% amino acid sequence homology with mouse and rat PCPE-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PCPE-1/PCOLCE Antibody (AF2627)(1)

Publication using AF2627

• Vadon-Le Goff, S;Tessier, A;Napoli, M;Dieryckx, C;Bauer, J;Dussoyer, M;Lagoutte, P;Peyronnel, C;Essayan, L;Kleiser, S;Tueni, N;Bettler, E;Mariano, N;Errazuriz-Cerda, E;Fruchart Gaillard, C;Ruggiero, F;Becker-Pauly, C;Allain, JM;Bruckner-Tuderman, L;Nyström, A;Moali, C; Identification of PCPE-2 as the endogenous specific inhibitor of human BMP-1/tolloid-like proteinases Nature communications 2023-12-04 [PMID: 38049428] (Western Blot, Mouse, Human)

Review for PCPE-1/PCOLCE Antibody (AF2627) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Protein simple using Jess device. Human 09/14/2021

Summary

• Application: Other
• Sample Tested: Heart lysates
• Species: Human
• Lot: XDZ01

Comments

• Comments: Use antibody at 10 fold dilution (saturation of mAb signal). Protein simple using Jess device, validation of antibody. ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

Bioinformatics

• Gene Symbol: PCOLCE
• Uniprot:
  • Q15113()