PCP4 Antibody (CL5306) - BSA Free

Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP2-61410] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.

Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP2-61410] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.

Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP2-61410] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.

Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP2-61410] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.

Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP2-61410] - Staining of mouse brain shows strong cytoplasmic positivity in Purkinje cells.

Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP2-61410] - Staining of rat brain shows strong cytoplasmic positivity in Purkinje cells.

• Reactivity: Hu, Mu, Rt
• Applications: IHC
• Clone: CL5306
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

PCP4 Antibody (CL5306) - BSA Free Summary

• Description: Novus Biologicals Mouse PCP4 Antibody (CL5306) - BSA Free (NBP2-61410) is a monoclonal antibody validated for use in IHC. Anti-PCP4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from P48539, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PCP4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  PCP4 Recombinant Protein Antigen (NBP2-61410PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for PCP4 Antibody (CL5306) - BSA Free

• brain specific polypeptide PEP19, 10Brain-specific antigen PCP-4
• Brain-specific polypeptide PEP-19
• PEP19
• PEP-19
• Purkinje cell protein 4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PCP4 Antibody (NBP2-61410)(1)

Publication using NBP2-61410

• Sanchez-Aguilera A, Wheeler DW, Jurado-Parras T et al. An update to Hippocampome.org by integrating single-cell phenotypes with circuit function in vivo PLoS biology 2021-05-01 [PMID: 33956790] (IF/IHC, Rat)

Review for PCP4 Antibody (NBP2-61410) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Verified Customer IHC – Free floating Rat 07/25/2018

Summary

• Application: Other
• Sample Tested: Cerebellum and hippocampal tissue sections
• Species: Rat
• Lot: 03167

Comments

• Comments: IF staining of rat cerebellum (A) and rat hippocampal CA2 region (B) with NBP2-61410 (red, left) and a polyclonal PCP4 antibody (green, middle). The panels on the right show the merged red and green channels. Staining was carried out without (upper row) or with antigen retrieval.

Bioinformatics

• Gene Symbol: PCP4