Recombinant Human PCNA associated factor His Protein

SDS-Page: PCNA associated factor Protein [NBP2-23168]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human PCNA associated factor His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-111 of Human PCNA associated factor
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMVRTKAD SVPGTYRKVV AARAPRKVLG SSTSATNSTS VSSRKAENKY AGGNPVCVRP TPKWQKGIGE FFRLSPKDSE KENQIPEEAG SSGLGKAKRK ACPLQPDHTN DEKE

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PCLAF
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 14.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human PCNA associated factor His Protein

• HCV NS5A-transactivated protein 9
• Hepatitis C virus NS5A-transactivated protein 9
• KIAA0101
• NS5ATP9p15PAF
• OEATC-1OEATC1
• Overexpressed in anaplastic thyroid carcinoma 1
• p15(PAF)
• PAF
• PCNA-associated factor

Background

KIAA0101 is a PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions. This protein also acts as a regulator of centrosome number. Recombinant human KIAA0101 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PCLAF