PCK2 Antibody (JB52-39)

Western Blot: PCK2 Antibody (JB52-39) [NBP2-75610] - Analysis of PCK2 on human kidney tissue (1) and MCF-7 cell (2) lysate using anti-PCK2 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: PCK2 Antibody (JB52-39) [NBP2-75610] - Staining PCK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PCK2 Antibody (JB52-39) [NBP2-75610] - Analysis of paraffin-embedded rat kidney tissue using anti-PCK2 antibody. Counter stained with hematoxylin.

Flow Cytometry: PCK2 Antibody (JB52-39) [NBP2-75610] - Analysis of MCF-7 cells with PCK2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: PCK2 Antibody (JB52-39) [NBP2-75610] - Staining PCK2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PCK2 Antibody (JB52-39) [NBP2-75610] - Analysis of paraffin-embedded human kidney tissue using anti-PCK2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PCK2 Antibody (JB52-39) [NBP2-75610] - Analysis of paraffin-embedded human liver tissue using anti-PCK2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PCK2 Antibody (JB52-39) [NBP2-75610] - Analysis of paraffin-embedded human colon cancer tissue using anti-PCK2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PCK2 Antibody (JB52-39) [NBP2-75610] - Analysis of paraffin-embedded mouse colon tissue using anti-PCK2 antibody. Counter stained with hematoxylin.

Product Discontinued

PCK2 Antibody (JB52-39) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within C-terminal Human PCK2 . (SwissProt: Q16822 Human; SwissProt: Q8BH04 Mouse; Entrez Gene: 361042 Rat)
• Localization: Mitochondrion.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PCK2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 71 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PCK2 Antibody (JB52-39)

• EC 4.1.1.32
• PE
• PEPCK
• PEPCK2PEP carboxykinase
• PEPCK-M
• phosphoenolpyruvate carboxykinase [GTP], mitochondrial
• phosphoenolpyruvate carboxykinase 2 (mitochondrial)
• Phosphoenolpyruvate carboxylase
• phosphopyruvate carboxylase

Background

PCK2 encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PCK2 Antibody (NBP2-75610)(1)

Publication using NBP2-75610

• Ko DS, Kang J, Heo HJ et al. Role of PCK2 in the proliferation of vascular smooth muscle cells in neointimal hyperplasia International journal of biological sciences 2022-08-08 [PMID: 35982907] (IHC-P, WB, ICC/IF, Mouse, Human)
  
  Details:
  IHC-P Dilutions: 1:200; ICC/IF Dilutions: 1:400; WB Dilutions: 1:1000

Bioinformatics

• Gene Symbol: PCK2