PCDHGA5 Antibody (5H5) - Azide and BSA Free

Western Blot: PCDHGA5 Antibody (5H5) [H00056110-M01] - Analysis of PCDHGA5 expression in transfected 293T cell line by PCDHGA5 monoclonal antibody (M01), clone 5H5.Lane 1: PCDHGA5 transfected lysate(100.935 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: PCDHGA5 Antibody (5H5) [H00056110-M01] - Detection limit for recombinant GST tagged PCDHGA5 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 5H5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PCDHGA5 Antibody (5H5) - Azide and BSA Free Summary

• Immunogen: PCDHGA5 (NP_061741, 205 a.a. ~ 304 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ETVHDLLLTALDGGDPVLSGTTHIRVTVLDANDNAPLFTPSEYSVSVPENIPVGTRLLMLTATDPDEGINGKLTYSFRNEEEKISETFQLDSNLGEISTL
• Specificity: PCDHGA5 - protocadherin gamma subfamily A, 5
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PCDHGA5
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PCDHGA5 Antibody (5H5) - Azide and BSA Free

• cadherin ME3
• CDH-GAMMA-A5
• ME3
• MGC142020
• PCDH-GAMMA-A5
• protocadherin gamma subfamily A, 5
• protocadherin gamma-A5

Background

This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PCDHGA5
• Entrez:
  • 56110(Human)
• OMIM:
  • 606292(Human)
• Uniprot:
  • NP_061741(Human)