PBXIP1 Antibody - BSA Free

Western Blot: PBXIP1 Antibody [NBP3-05982] - Lane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:81 kDaObserved band size:97 kDa

Immunohistochemistry-Paraffin: PBXIP1 Antibody [NBP3-05982] - Staining of human PBXIP1 in human skeletal muscle with rabbit polyclonal antibody at 1:2000 dilution.

Immunohistochemistry-Paraffin: PBXIP1 Antibody [NBP3-05982] - Staining of human PBXIP1 in human stomach with rabbit polyclonal antibody at 1:2000 dilution.

Immunoprecipitation: PBXIP1 Antibody [NBP3-05982] - Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-PBXIP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PBXIP1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 81 kDaObserved band size :97 kDa

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PBXIP1 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human PBXIP1 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PBXIP1
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1-5uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for PBXIP1 Antibody - BSA Free

• Hematopoietic PBX-interacting protein
• HPIPpre-B-cell leukemia transcription factor interacting protein 1
• pre-B-cell leukemia homeobox interacting protein 1
• pre-B-cell leukemia transcription factor-interacting protein 1

Background

Hematopoietic PBX-interacting protein (HPIP) was identified in a yeast two-hybrid screen for factors that interact with PBX1, a homeodomain protein that regulates gene transcription during development and differentiation. Recently, HPIP has been reported to interact with estrogen receptor alpha (ERalpha) and estrogen receptor beta (ERbeta) and modulate ER target gene expression. Alternate names for HPIP include pre-b-cell leukemia transcription factor-interacting protein 1, hematopoietic PBX-interacting protein, and PBKIP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PBXIP1