PBK Antibody - BSA Free

Western Blot: PBK Antibody [NBP3-12989] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PBK antibody (NBP3-12989) diluted at 1:60000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: PBK Antibody [NBP3-12989] - PBK antibody detects PBK protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PBK protein stained by PBK antibody (NBP3-12989) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Western Blot: PBK Antibody [NBP3-12989] - PBK antibody detects PBK protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PBK antibody (NBP3-12989) diluted at 1:1000.

Western Blot: PBK Antibody [NBP3-12989] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PBK antibody (NBP3-12989) diluted at 1:1000.

Immunoprecipitation: PBK Antibody [NBP3-12989] - Immunoprecipitation of PBK protein from A431 whole cell extracts using 5 ug of PBK antibody (NBP3-12989). Western blot analysis was performed using PBK antibody (NBP3-12989) diluted at 1:500. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

• Reactivity: Hu, Mu, Rt, Po, Bv, Pm
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PBK Antibody - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PBK. The exact sequence is proprietary.
• Predicted Species: Mouse (90%), Rat (93%), Porcine (93%), Chimpanzee (99%), Bovine (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PBK
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for PBK Antibody - BSA Free

• Cancer/testis antigen 84
• CT84MAPKK-like protein kinase
• EC 2.7.12.2
• FLJ14385
• Nori-3PDZ-binding kinase
• PDZ binding kinase
• serine/threonine protein kinase
• Spermatogenesis-related protein kinase
• SPKT-LAK cell-originated protein kinase
• TOPKlymphokine-activated killer T-cell-originated protein kinase

Background

PBK encodes a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PBK