Paxillin Antibody (S04-3C3)

Western Blot: Paxillin Antibody (S04-3C3) [NBP3-20046] - Western blot detection of Paxillin in C6 cell lysates using NBP3-20046 (1:1000 diluted). Predicted band size: 65kDa. Observed band size: 65kDa.

Immunohistochemistry-Paraffin: Paxillin Antibody (S04-3C3) [NBP3-20046] - Immunohistochemistry of Paxillin in paraffin-embedded Human breast cancer tissue using NBP3-20046 at dilution 1/50

Immunocytochemistry/Immunofluorescence: Paxillin Antibody (S04-3C3) [NBP3-20046] - Immunofluorescence of Paxillin (green) in hela using NBP3-20046 at dilution 1/5, and DAPI (blue)

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: S04-3C3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

Paxillin Antibody (S04-3C3) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human Paxillin (Uniprot # P49023)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PXN
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1/50
  • Immunohistochemistry 1/20-1/100
  • Immunohistochemistry-Paraffin 1:20-1:100
  • Western Blot 1/1000-1/5000
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for Paxillin Antibody (S04-3C3)

• FLJ16691
• Paxillin
• PXN

Background

Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) (1). Paxillin plays a significant role in integrin signaling and the tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118 (3). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PXN