Paxillin Antibody (LD4 sAB) - Chimeric - Azide and BSA Free

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: LD4 sAB
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Paxillin Antibody (LD4 sAB) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Paxillin Antibody (LD4 sAB) - Chimeric - Azide and BSA Free (NBP2-81063) is a recombinant monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Peptide corresponding to human paxillin-LD4 motif (residues 260-278) fused to MBP; peptide sequence: WGGSATRELDELMASLSD.
• Specificity: The antibody binds specifically to the LD-4 motif of human Paxillin with a Kd of 6 nM.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PXN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques. This antibody binds to the LD4-motif of human paxillin, a signal transduction adaptor protein involved in focal adhesion, with binding partners including integrins, Src, FAK or actin complexes. The antibody inhibits complex formation between paxillin and FAK. Paxillin is involved in both inside-out cell signalling and outside-to-inside cell signalling.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Paxillin Antibody (LD4 sAB) - Chimeric - Azide and BSA Free

• FLJ16691
• Paxillin
• PXN

Background

Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) (1). Paxillin plays a significant role in integrin signaling and the tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118 (3). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PXN