Paxillin Antibody (LD4 sAB) [HRP]

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: LD4 sAB
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: HRP

Paxillin Antibody (LD4 sAB) [HRP] Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Peptide corresponding to human paxillin-LD4 motif (residues 260-278) fused to MBP; peptide sequence: WGGSATRELDELMASLSD.
• Specificity: The antibody binds specifically to the LD-4 motif of human Paxillin with a Kd of 6 nM.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PXN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Paxillin Antibody (LD4 sAB) [HRP]

• FLJ16691
• Paxillin
• PXN

Background

Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) (1). Paxillin plays a significant role in integrin signaling and the tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118 (3). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PXN